FFPE DNA Hloekiso Kit

FFPE DNA Purification Kit Featured Image

Tlhaloso e Khutšoanyane:

Setsi sena se sebelisa tharollo ea dewaxing ea tikoloho ho ntša DNA ea boleng bo phahameng ea genomic ho tloha lisampoleng tse kentsoeng ka parafine, ho hlōla phello ea thibelo e bakoang ke formalin cross-linking.
Mohlala o kopantsoe le cell lysis buffer ho pshatla lisele le ho pepesa DNA. Mohlala o kopantsoe le cell lysis buffer ho senya lisele le ho pepesa DNA. DNA e pepesitsoeng e nkoa ke magnetic bead nanoparticles ho etsa "bead-DNA complex". Lifaha-DNA complexes li fetisetsoa ho Lifaha-DNA complexes li fetisetsoa ho hlatsoa buffer ho tlosa litšila tse feteletseng. Qetellong, DNA ea hlakoloa le ho khutlisoa ka ho e fetisetsa sebakeng sa elution buffer.

ITEANYE LE RONA

Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Mehlala

Likarolo tsa FFPE, li-blocks tsa FFPE, sampole ka tharollo ea ho lokisa joalo ka formalin

Likaroloana

Mohlala	BFMP12R1	BFMP12R16	BFMP12R	Likaroloana
Ho paka	1T40T/kit	16T16T/kit	32T32T/kit
Protease K	400μL x 2	320μL	640μL	Tharollo ea protease (E pakiloe ka thoko)
RNase A	80μL	32μL	64μL	Tharollo ea enzyme (E pakiloe ka thoko)
Buffer FA	12mL	5ml	10ml	Tharollo e phahameng ea letsoai
Buffer FL	Ka mashome a mane a metso e tšeletseng hantle prep. khatriji lik'hilograma tse 40.	96 hantle e phuthetsoe pele poleiti 2 likhomphutha	96 hantle e phuthetsoe pele poleiti 2 likhomphutha.	Denaturant e matla le buffer ea Tris
Buffer WA				Tharollo e phahameng ea letsoai
Buffer WB				Tharollo e tlaase ea saline
Buffer DE				Tharollo e tlaase ea saline
Lifaha tsa makenete				Hydroxy magnetic bead solution
Bukana ea Basebelisi	1	1	1