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Sete ea ho hloekisa DNA ea genomic ea Magapure FFPE
Selelekela se sekhutšoanyane
Sete ena e amohela sistimi e ikhethang e ntlafalitsoeng le e ntlafalitsoeng ea buffer le lifaha tsa makenete tse tlamang ka ho khetheha ho DNA, tse ka tlamang, tsa monya, tsa arola le ho hloekisa li-nucleic acid ka potlako. Mokhoa o khethehileng oa ho qhaqha mafome o sebelisoa ho petsola le ho lokolla DNA likarolong tsa lisele, ho fokotsa kotsi ea tšenyo ho DNA e bakoang ke ho hokahana ha liketane tsa limolek'hule tse bakoang ke lisele tse tsitsitseng tsa formalin. Ka ho tšehetsa tšebeliso ea Bigfish Magnetic Bead Nucleic Acid Extractor, e loketse haholo bakeng sa ho ntša ka boiketsetso boholo ba lisampole tse kholo. DNA ea genomic e ntšitsoeng e na le bohloeki bo phahameng le boleng bo botle, 'me e ka sebelisoa haholo lipatlisisong tse tlase tsa PCR/qPCR, NGS le lipatlisiso tse ling tsa liteko.
Likarolo tsa sehlahisoa
◆E bolokehile ebile e ka tšeptjoa: E sebelisa mokelikeli o tlosang litšila o sireletsang tikoloho, ha e sebelise metsoako ea tlhaho e kang xylene, 'me ha e na chefo ebile ha e kotsi.
◆E potlakile ebile e bonolo: Mokhoa oa makenete o sebelisoa bakeng sa ho ntša le ho hloekisa, ka tekanyo e phahameng ea othomathike 'me ha ho hlokahale hore ho sebelisoe centrifugation ea mehato e mengata.
◆Boleng bo botle: DNA ea genomic e ntšitsoeng e na le mahloriso a phahameng, bohloeki le botšepehi, 'me e ka sebelisoa ka ho toba bakeng sa liteko tse latelang.
Sesebelisoa se ikamahanyang le maemo
Tlhapi e Kholo BFEX-32/BFEX-32E/BFEX-96E
Litekanyetso tsa tekheniki
Bophahamo ba sampole: dilae tse 5-8 tsa 5-10 μm
Bohloeki ba DNA: A260/280≧1.75
Tlhaloso ea sehlahisoa
| Lebitso la Sehlahisoa | Katse. Tjhe. | Ho paka |
| MakasineaE hloekileFFPE GenomicKiti ea ho Hloekisa DNA(psephutheloana se tlatsitsoeng hape) | BFMP12R | 32T |
| MakasineaE hloekileFFPE GenomicKiti ea ho Hloekisa DNA (sephutheloana se tlatsitsoeng esale pele) | BFMP12R1 | 40T |
| MakasineaE hloekileFFPE GenomicKiti ea ho Hloekisa DNA (sephutheloana se tlatsitsoeng esale pele) | BFMP12R96 | 96T |
| RNase A(pthepa e rekiloeng) | BFRD017 | 1ml/phaephe (10mg/ml) |
Litlhophiso tsa lekunutu