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Kiti ea ho Hloekisa DNA ea MagaPure ea Liphoofolo tsa Metsing
Selelekela se sekhutšoanyane
Sete ena e amohela sistimi e ikhethang e ntlafalitsoeng le e ntlafalitsoeng ea buffer le lifaha tsa makenete tse tlamang DNA ka ho khetheha. E ka tlama, ea monya, ea arola le ho hloekisa li-nucleic acid ka potlako, 'me e etselitsoe liphoofolo tsa metsing ka ho khetheha. E loketse haholo ho ntša le ho hloekisa DNA ea genomic ka katleho liseleng tse fapaneng tsa liphoofolo tsa metsing, 'me e ka tlosa litšila tse kang liprotheine, mafura le metsoako e meng ea tlhaho ka hohle kamoo ho ka khonehang. Ka ho tšehetsa tšebeliso ea Bigfish Magnetic Bead Nucleic Acid Extractor, e loketse haholo ho ntša ka boiketsetso boholo ba sampole e kholo. DNA ea genomic e ntšitsoeng e na le bohloeki bo phahameng le boleng bo botle, 'me e ka sebelisoa haholo lipatlisisong tse tlase tsa PCR/qPCR, NGS, Southern hybridization le lipatlisiso tse ling tsa liteko.
Likarolo tsa sehlahisoa
◆ Disampole tse sebetsang haholo: DNA ea genomic e ka ntšoa ka ho toba lisampoleng tse fapaneng tsa liphoofolo tsa metsing.
◆ E bolokehile ebile ha e na chefo: Reagent ha e na metsoako e chefo e kang phenol le chloroform, e nang le ntlha e phahameng ea polokeho.
◆ Boiketsetso: E na le Bigfish Nucleic Acid Extractor, e ka etsa tlhahiso e phahameng ea ho ntša, haholo-holo e loketseng ho ntša boholo bo boholo ba sampole
◆ Bohloeki bo phahameng: bo ka sebelisoa ka kotloloho bakeng sa liteko tsa baeloji ea limolek'hule tse kang PCR, tšilo ea enzyme, ho kopanya, jj.
Mekhoa ea ho ntša metsi
Ho etsa sampole: Nka 25-30 mg ea lisele tsa liphoofolo tsa metsing
Ho sila: ho sila ka naetrojene e metsi, ho sila ka grinder kapa ho kuta
Tšilo ea lijo: 56℃ tšilo ea lijo e futhumetseng ea bateng
Mochining: Kenya supernatant ka centrifuge ebe o e eketsa poleiting bakeng sa ho ntša
Litekanyetso tsa tekheniki
Sampole ea sage: 25-30mg
Bohloeki ba DNA: A260/280≧1.75
Sesebelisoa se ikamahanyang le maemo
Tlhapi e Kholo BFEX-32/BFEX-32E/BFEX-96E
Tlhaloso ea sehlahisoa
| Lebitso la Sehlahisoa | Katse. Tjhe. | Ho paka |
| MakasineaE hloekileGenomic ea Liphoofolo tsa MetsingKiti ea ho Hloekisa DNA(psephutheloana se tlatsitsoeng hape) | BFMP21R | 32T |
| MakasineaE hloekileGenomic ea Liphoofolo tsa MetsingKiti ea ho Hloekisa DNA (sephutheloana se tlatsitsoeng esale pele) | BFMP21R1 | 40T |
| MakasineaE hloekileGenomic ea Liphoofolo tsa MetsingKiti ea ho Hloekisa DNA (sephutheloana se tlatsitsoeng esale pele) | BFMP21R96 | 96T |
| RNase A(pthepa e rekiloeng) | BFRD017 | 1ml/phaephe (10mg/ml) |
Litlhophiso tsa lekunutu