Kiti ea ho Hloekisa DNA ea MagaPure Oryza sativa L. Genomic

Tlhaloso e Khutšoanyane:

Sete ena e amohela sistimi e ikhethang e ntlafalitsoeng le e ntlafalitsoeng ea buffer le lifaha tsa makenete tse tlamang ka ho khetheha ho DNA, e ka tlamang, ea monya, ea arola le ho hloekisa li-nucleic acid ka potlako, ha ka nako e ts'oanang e tlosa litšila tse kang li-polysaccharides le li-polyphenol complexes limela. E loketse haholo ho ntša DNA ea genomic liseleng tsa makhasi a limela. Ka ho tšehetsa ts'ebeliso ea Bigfish Magnetic Bead Nucleic Acid Extractor, e loketse haholo ho ntša ka boiketsetso boholo ba sampole. DNA ea genomic e ntšitsoeng e na le bohloeki bo phahameng le boleng bo botle, 'me e ka sebelisoa haholo lipatlisisong tse tlase tsa PCR/qPCR, NGS le lipatlisiso tse ling tsa liteko.

ITEANYE LE RONA

Qaqiso ea Sehlahisoa

Li-tag tsa Sehlahisoa

Selelekela se sekhutšoanyane

Likarolo tsa sehlahisoa

◆ E bolokehile ebile ha e na chefo: Ha ho hlokahale li-reagent tse chefo tsa tlhaho tse kang phenol/chloroform
◆ E iketsahalletseng ka ho fetisa matla a phahameng: E na le Bigfish Nucleic Acid Extractor, e ka ntša matla a phahameng a ho fetisa matla a mangata mme e loketse ho ntša boholo bo boholo ba sampole.
◆ Bohloeki bo phahameng le boleng bo botle: Sehlahisoa se ntšitsoeng se na le bohloeki bo phahameng 'me se ka sebelisoa bakeng sa NGS e ka tlase, ho kopanya li-chip le liteko tse ling.

Mekhoa ea ho ntša metsi

Ho etsa sampole: Sampole e ncha e ka bang 100 mg kapa sampole ea boima bo omileng e ka bang 30 mg
Ho sila: Sila ka botlalo ka naetrojene e metsi kapa grinder
Tšilo ea lijo: 65℃ tšilo ea lijo e futhumetseng ea bateng
Mochining: Kenya supernatant ka centrifuge ebe o e eketsa poleiting bakeng sa ho ntša

Sesebelisoa se ikamahanyang le maemo

Tlhapi e Kholo BFEX-32/BFEX-32E/BFEX-96E

Tlhaloso ea sehlahisoa

Lebitso la Sehlahisoa	Katse. Tjhe.	Ho paka
MakasineaE hloekileOryza sativa L.Kiti ea ho Hloekisa DNA ea Genomic(psephutheloana se tlatsitsoeng hape)	BFMP23R	32T
MakasineaE hloekileOryza sativa L.Kiti ea ho Hloekisa DNA ea Genomic (sephutheloana se tlatsitsoeng esale pele)	BFMP23R96	96T
Protheine K (p)thepa e rekiloeng)	BFRD007	1ml/phaephe (10mg/ml)
RNase A(pthepa e rekiloeng)	BFRD017	1ml/phaephe (10mg/ml)