MagPure Plasmid DNA Purification Kit
Kenyelletso e khutšoanyane
Setsi sena se amohela sistimi e ikhethileng e ntlafalitsoeng le e ntlafalitsoeng ea buffer le lifaha tsa makenete tse tlamahanyang DNA ka ho khetheha, tse ka tlamang, tsa bapatsa, tsa arola le ho hloekisa li-nucleic acid. E loketse haholo ho arola le ho hloekisa DNA ea plasmid ka potlako le ka katleho ho tloha ho 0.5-2mL (ka tloaelo 1-1.5mL) mokelikeli oa baktheria, ha o ntse o tlosa mesaletsa e kang liprotheine le li-ion tsa letsoai. Ka ho ts'ehetsa ts'ebeliso ea Bigfish Magnetic Bead Nucleic Acid Extractor, e loketse haholo bakeng sa ho ntšoa ka boiketsetso ba boholo ba sampole. DNA e nkiloeng ea plasmid e na le bohloeki bo phahameng le boleng bo botle, 'me e ka sebelisoa haholo litekong tse ka tlaase ho metsi tse kang tšilo ea enzyme, ligation, phetoho, NGS, joalo-joalo..
Likarolo tsa Sehlahisoa
Boleng bo botle:Arola le ho hloekisa DNA ea plasmid ho tsoa ho tharollo ea baktheria ea 0.5-2mL e nang le chai e ngata le bohloeki bo botle..
Kapele le bonolo:Ts'ebetso eohle ha e hloke ts'ebetso e pheta-phetoang ea centrifugation kapa filtration, e etsa hore e lokele ho ntša boholo ba sampole..
E bolokehile ebile ha e na chefo:Ha ho na tlhoko ea li-reagents tse chefo tse kang phenol/chloroform.
E ka fetohaLisebelisoa
Tlhapi e kgolo: BFEX-32E, BFEX-32, BFEX-96E, BFEX-16E
Tlhaloso ea sehlahisoa
SehlahisoaName | Katse. Che. | Ho paka |
MagPure Plasmid DNA Purification Kit (sephutheloana se tlatsitsoeng pele) | BFMP09R | 32T |
MagPure Plasmid DNA Purification Kit (sephutheloana se tlatsitsoeng pele) | BFMP09R1 | 40T |
MagPure Plasmid DNA Purification Kit (sephutheloana se tlatsitsoeng pele) | BFMP09R96 | 96T |
RNaseA(reka) | BFRD017 | 1 ml / tube(10mg/ml) |
