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Raese ke e 'ngoe ea lijalo tsa bohlokoa ka ho fetisisa, tse leng tsa limela tsa litlama tsa metsing tsa lelapa la Poaceae. Chaena ke e 'ngoe ea libaka tsa pele tsa bolulo ba raese, tse lengoang haholo karolong e ka boroa ea Chaena le sebakeng se ka Leboea-bochabela. Ka tsoelo-pele ea saense le theknoloji, mekhoa ea sejoale-joale ea baeloji ea limolek'hule e sebelisoa haholo lipatlisisong tsa raese. Ho fumana DNA ea genomic ea raese ea boleng bo holimo, e hloekileng haholo ho beha motheo o tiileng bakeng sa lithuto tsa liphatsa tsa lefutso tse theohelang. Sete ea BigFish Sequence Magnetic Bead-Based Rice Genomic DNA Purification Kit e nolofalletsa bafuputsi ba raese ho ntša DNA ea raese habonolo, kapele le ka katleho.
Kiti ea ho Hloekisa DNA ea Genome ea Raese
Kakaretso ea Sehlahisoa:
Sehlahisoa sena se sebelisa sistimi e ikhethang ea buffer e ntlafalitsoeng ka ho khetheha le e ntlafalitsoeng le lifaha tsa makenete tse nang le thepa e itseng ea ho tlama DNA. Se tlama, se monye, 'me se arole li-nucleic acid ka potlako ha se ntse se tlosa litšila tse kang li-polysaccharides le metsoako ea polyphenolic ho tsoa limela ka katleho. Se loketse haholo bakeng sa ho ntša DNA ea genomic ho tsoa liseleng tsa makhasi a limela. Ha se kopantsoe le BigFish Magnetic Bead Nucleic Acid Extraction Instrument, se loketse ho ntša ka boiketsetso li-sampole tse kholo. Lihlahisoa tsa nucleic acid tse ntšitsoeng li bontša bohloeki bo phahameng le boleng bo botle, e leng se etsang hore li sebelisoe haholo bakeng sa lipatlisiso tsa liteko tse tlase joalo ka PCR/qPCR le NGS.
Likarolo tsa Sehlahisoa:
E bolokehile ebile ha e na chefo: Ha ho hlokahale li-reagent tse chefo tsa tlhaho tse kang phenol/chloroform
E iketsahalletseng ka ho fetisa matla a phahameng: E kopantsoe le Beagle sequencing nucleic acid extractor, e ka ntša matla a phahameng a ho fetisa matla a mangata 'me e loketse ho ntša boholo bo boholo ba lisampole.
Bohloeki bo phahameng le boleng bo botle: Sehlahisoa se ntšitsoeng se na le bohloeki bo phahameng 'me se ka sebelisoa bakeng sa NGS e ka tlase, ho kopanya li-chip le liteko tse ling.
Lisebelisoa tse lumellanang: BigFish BFEX-32/BFEX-32E/BFEX-96E
Nako ea poso: Loetse-11-2025
Litlhophiso tsa lekunutu