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Masimong atlhahlobo ea kliniki ea in vitro (IVD), genotyping, le lipatlisiso tsa limolek'hule, disampole tsa molomo—tse kangli-swab tsa molomo, li-swab tsa 'metso, le mathe—li sebelisoa haholo bakeng sa liteko tsa nucleic acid ka lebaka la tsonapokello e bonolo, tlhaho e sa hlaseleng, le ts'ebetso ea ho nka lisampole e se nang bohlokoLeha ho le jwalo, disampole tsa molomo hangata di na letekanyo e fokolang ea li-nucleic acid'me hangata li silafalitsoe keliprotheine le litšila tse lingMekhoa ea setso ea ho ntša metsi hangata e na le bothata bamekhoa e rarahaneng ea mosebetsi, bokhoni bo tlase, le tšebeliso ea li-reagent tse chefo, e leng se ka behang kotsing e kgolo ho nepahala le botsitso ba ditshebediso tse latelang jwalo kaPCR/qPCR le tatellano ea moloko o latelang (NGS).
TheSete ea ho ntša DNA ea Genomic DNA e thehiloeng ho Magnetic Bead ea BFMP06, e ntlafalitsoe keTheknoloji ea Baeloji ea Hangzhou Bigfish FeiXu, e fana katharollo e bolokehileng, e sebetsang hantle le e tšepahalangbakeng sa ho ntšoa ha DNA ka sampole ka molomo. Ka moralo oa eona o mocha oa tekheniki le maemo a thata a ts'ebetso, kit ena e fetohile sesebelisoa se tšepahalang bakeng sa lilaboratori tsa bongaka le tsa lipatlisiso.
Kiti ea BFMP06 e hahiloe ho potolohaSistimi ea buffer e ntlafalitsoeng ka mokhoa o ikhethange kopantsoe leLifaha tsa makenete tsa hydroxyl tse ikhethileng tsa DNA, ho theha mokhoa o sebetsang hantle oa ho hloekisa nucleic acid. Kamora hore sampole e hlahlojoe ka har'a lysis buffer, likarolo tsa sele lia sitisoa 'me li-nucleic acid lia lokolloa. Lihlopha tse sebetsang holim'a lifaha tsa makenete li tlama DNA e lokolohileng ka khetho, li etsa botsitso.metsoako ea magnetic bead–DNA.
Tlas'a matla a makenete a kantle, li-complexes li fetamehato e 'meli e nepahetseng ea ho hlatsoaho tlosa liprotheine, letsoai le litšila tse ling ka botlalo. Qetellong,DNA ea genomic e hloekileng haholoe phonyoha ka katleho ho sebelisoa sesebelisoa sa ho thibela ho ruruha.
Tlhaloso ea Sehlahisoa
Sehlahisoa sena se sebelisa sistimi ea buffer e ntlafalitsoeng ka ho khetheha le e ntlafalitsoeng hammoho lelifaha tsa makenete tse tlamang DNA ka ho khetheha, e nolofalletsang ho monya, ho arola le ho hlwekisa di-nucleic acid ka potlako. E loketse haholo bakeng saho aroloa ha DNA ea genomic ka potlako le ka katleho ho tsoa ho li-swab tsa molomo, li-swab tsa 'metso le lisampole tsa mathe, ha ka nako e ts'oanang e tlosa liprotheine le letsoai tse setseng ka katleho.
Ha e sebelisoa hammoho leLisebelisoa tsa ho ntša nucleic acid tse thehiloeng holim'a Bigfish FeiXu tse thehiloeng holim'a bead ea makenete, kit ena e loketse bakeng saho ntša ka boiketsetso ka mokhoa o phahamengDNA e hloekisitsoeng ea genomic ke eabohloeki bo phahameng le boleng bo botle haholo, e leng se etsang hore e lokele mefuta e mengata ea lits'ebetso tse theohelang tlase, ho kenyeletsoaPCR/qPCR le NGS.
Likarolo tsa Sehlahisoa
Boleng bo phahameng
E arola le ho hloekisa DNA ea genomic ka katleho holi-swab tsa molomo, li-swab tsa 'metso, le mathe, ho fana kachai e ngata le bohloeki bo phahameng.
E Potlakile ebile e le bonolo
Ha ho hlokahale mehato ea ho centrifugation e pheta-phetoang kapa ho sefa ka vacuum. E lumellana le lisebelisoa tsa ho ntša tse iketsang, e leng se etsang hore e be ntle bakeng sats'ebetso e kholo ea sampole.
E Sireletsehile ebile ha e na chefo
Ha ho hlokahale li-reagent tse chefo tsa tlhaho tse kangphenol kapa chloroform.
Lisebelisoa tse Lumellanang
Tlhapi e Kholo FeiXu BFEX-16E
BFEX-32
BFEX-32E
BFEX-96
Liphetho tsa Liteko
Disampole tsa swab ya molomo (tse qoelisoang ka hare hoTharollo ea poloko ea 400 μL) le disampole tsa mathe (Tharollo ea ho boloka mathe ea 200 μL + 200 μL) li sebetsoa ka ho sebelisaBigfish FeiXu Oral Swab Genomic DNA purification KitDNA e ile ea qojoa kaBuffer ea tharollo ea 70 μLle ho hlahlojoa keelectrophoresis ea gel ea agarose, joalo ka ha ho bontšitsoe setšoantšong se ka tlase.
M: Letshwao la DNA (2K Plus II)
Litlhaloso tsa Sehlahisoa
Nako ea poso: Pherekhong-15-2026
Litlhophiso tsa lekunutu