Lekola ho fapanyetsana ha meqomo ea li-earmal ho etsa lipatlisiso

Li-Crocker tsa li-turmal, li boetse li tsejoang ka hore e se e le mechini ea PCR, ke lisebelisoa tsa bohlokoa tsa biology tsa bioggy le lipatlisiso tsa mofuta oa liphatsa tsa lefutso. Lisebelisoa tsena li sebelisetsoa ho holisa DNA le Rna ka polymerase Chain Recence (PR) theknoloji. Leha ho le joalo, ho feto-fetoha ha makhoaba a li-armal ha ho felle feela ho likopo tsa pcr. Sehloohong sena, re tla hlahloba metšoasong e fapaneng ea batho ba nang le bothata ba ho hlahisa tsebo ea saense.

1. Khohlo ea PCR

Ts'ebetso ea mantlha ea aCycle ea Thermalke ho etsa theknoloji ea pcc, e leng eona e bohlokoa bakeng sa lits'ebetso tse fapaneng tsa biog rur. Ka ho beha sampole ea DNA kapa RNA kapa Rna Ts'ebetso ena e bohlokoa bakeng sa tlhahlobo ea liphatsa tsa lefutso, ho na le lithuto tsa mofuta oa Gene, le ho lemoha basebeletsi ba tšoaetsanoang.

2. Comarmatimaticaticaties PCR (QPCR)

Ntle le PCR e tloaelehileng ea PCR e sebelisoa bakeng sa bongata kapa QPCCR, e lumella lipheo tsa ho qhalana ha liphokotso tsa nyutlelie. Ka ho kenyelletsa daes kapa lipono tsa fluorescent, metsu ea li-curmal e ka lekanya lintho tse bokelloang tsa pc ka nako ea 'nete, ho fana ka leseli la bohlokoa maemong a puo ea ge gene, le mefuta ea vaerase le phapang ea lefutso.

3. Reverse Transcent pr (RT-PR)

Li-Compers tsa li-turmal li phetha karolo ea bohlokoa ho ngollatsi ea sehlooho PRS. Mokhoa ona o bohlokoa bakeng sa ho ithuta puo ea Gene, vaerase ea Rna, le mrna splicing patterns. Cycler cycler e nang le taolo e nepahetseng ea mocheso e bohlokoa ka katleho ea liteko tsa rt-pcr.

4. Digital pcr

Tsoelo-pele ea Theknoloji ea Thermal crcle e lebisitse kholisong ea dijithale ea dijithale, e leng mokhoa oa tlhokomelo bakeng sa litlatsetso tsa nyutlelie. Ka ho arola karabelo ea PCR ka micro e le 'ngoe, likompitsi tsa li-harmal li ka etsa sesebelisoa sa pele sa ho lemoha le ho kopitsa nomoro ea phapang.

5. Litokisetso tsa lilaebrari tsa meroho tse latelang

Li-cyclers tsa li-turmal ke karolo ea bohlokoa ea ts'ebetso ea laebrari bakeng sa lits'ebetso tse latelang tsa likotlo tse latelang. Ka ho etsa phetisetso ea DNA e thehiloeng ho PRNAL, li-cyclers tsa li-formal li thusa kaho ea li-lilaebrari tse sa tsotelleng, tse lumellanang le bafuputsi ba libini, engoli, kapa epigenome.

6. Proption boenjiniere le musgesis

Ntle le ho holisa ha nyutleiic acid, likokoana-hloko tsa li-turmal li sebelisoa ho li-boenjiniere ba protheine le liphuputso tsa bolebele. Lisebelisoa tse tataiselitsoeng sebakeng sa marang-rang, ho iphetola hoa protheine, le liteko tsa ntlafatso tsa ho iphelisa hangata le ho futhumatsa ho futhumatsa le ho futhumatsa ho bohlokoa ho fumana liphetho tse nepahetseng le tse khothatsang.

7. Tlhahlobo ea polokeho ea tikoloho le ea lijo

Makholo a li-armal le tsona li sebelisoa ka tlhahlobo ea polokeho ea tikoloho le ea lijo, ka ho khetheha ho aloa ha likokoana-hloko tsa likokoana-hloko, tse fetotsoeng liphatsa tsa likokoana-hloko tsa lijo. Liteko tse thehiloeng ho PCR li tsamaisoa ka li-croclers tsa PRMR li na le khethollo e potlakileng le e tobileng ea litlokotsebe, e netefatsa polokeho le boleng ba disampole tsa lijo le boleng ba lijo.

Ka kakaretso,Li-Cyclers tsa Thermalke lisebelisoa tsa bohlokoa tsa thuto ea limolek'hupelo le lipatlisiso tsa mofuta oa liphatsa tsa lefutso, ho fana ka lits'ebetso tse fapaneng tse fetang theipi e tloaelehileng ea P%. Ho fokola le ho halala le ketsahalo ea bona ho ba bohlokoa bakeng sa liteko tsa bona ho tsoa tlhahlobo ea nakoana ea ho lekola tikoloho ea Vepe. Joalo ka ha theknoloji e ntse e tsoela pele, metsu ea li-charmal e ka bapala karolo ea bohlokoahali ho khanna ho sibolla saense le ntlafatso.