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Tsoelopele e amanang
Ho ea ka Ofisi ea Boitsebiso ea Lekala la temo le libaka tsa mahaeng, ka Phato 2018, lefu la seoa la likolobe tsa Afrika le etsahetse Seterekeng se Secha sa Shenbei, Motse oa Shenyang, Profinseng ea Liaoning, e leng lefu la seoa la pele la likolobe tsa Afrika Chaena. Ho tloha ka la 14 Pherekhong 2019, lefu la seoa la likolobe tsa Afrika le etsahetse liprofinseng tse fetang 20 tsa China, le bolaile likolobe tse 916 000, tse bakang matšoenyeho a sechaba.
Letshoroma la kolobe la Afrika (ASF)
ASF(African Swine fever) ke lefu le tšoaetsanoang le matla le bakoang ke kokoana-hloko ea African swine fever (ACFV) e tšoaetsang likolobe tse ruuoang lapeng le likolobe-moru (likolobe tse hlaha tsa Afrika, likolobe tse hlaha tsa Europe, joalo-joalo). Mokhatlo oa lefats'e oa bophelo bo botle ba liphoofolo (OIE) o bo thathamisitse e le lefu le tlalehiloeng ka molao, leo hape e leng mofuta oa seoa sa liphoofolo seo China e shebaneng le ho se thibela.
Litšobotsi tsa lefu lena
Lipontšo tsa kliniki ke feberu (ho fihlela ho 40 ~ 42 ℃), tachycardia, dyspnea, ho khohlela ha karolo, serous kapa mucinous purulent secretion mahlong le nko, cyanosis ea letlalo, ho tsoa mali ho totobetseng liphio, lymph node le mucosa ea ka mpeng. Matshwao a kliniki a African classical swine fever a tshwana le a classical swine fever, a ka fumanwang feela ka tlhahlobo ya laboratori.
Tharollo ho African classical swine fever
1. Mohlala oa ho sebetsa
E loketse mali le lisele tse fapaneng tsa likolobe tse belaelloang tse kulang: spleen, lymph node le lisele tsa liphio.
Mehlala ea mali
Nka 200 μ L sampole ea mali, 5000g centrifugal 5 min, nka supernatant bakeng sa tlhahlobo.
Mehlala ea lisele
Ka mor'a hore lisampole tsa linama li theohe ka ho feletseng, ho ile ha eketsoa tekanyo e loketseng ea saline e tloaelehileng kapa PBS, 'me supernatant e ile ea kenngoa centrifuged hore e hlahlojoe.
2. Ho ntšoa ka mokhoa o itekanetseng
Hangzhou Bigfish Bio-tech Co., Ltd. BFEX-32 automatic nucleic acid extractor e ka qeta ho ntšoa ha disampole tse 32 ka metsotso e 30, e ka fihlelang litlhoko tsa hau tsa ho ntša lisampole tse ngata. Ts'ebetso eohle ha e hloke ts'ebetso e 'ngoe, e boloka nako ebile e fokotsa phoso ea ts'ebetso ea matsoho, mme e ntlafatsa ts'ebetso ea mosebetsi ka hohle kamoo ho ka khonehang.
3. Bohloeki bo phahameng ba nucleic acid bohloeki
MagPure Nucleic acid extract kit, e nang le BFEX-32, sehlahisoa se tsamaellana ka botlalo le ho fumanoa ha PCR & QPCR.
4. Teko ea khomphutha le tlhahlobo
Ho latela boemo ba 'nete ba laboratoring, mosebelisi o amohela leano la tlhahlobo ea boleng kapa palo.
Hangzhou Bigfish Bio-tech Co., Ltd. e fana ka lisebelisoa tsa ho lemoha tsa boleng (Bongata) bakeng sa African classical swine fever (ACFV) e nang le FC-96G (BFQP-16/48), e ka lemohang ACFV haholo, ka hloko le ka botšepehi.
Litaba tse ling, ka kopo ela hloko ak'haonte ea semmuso ea WeChat ea Hangzhou Bigfish Bio-tech Co., Ltd.
Nako ea poso: May-23-2021
Litlhophiso tsa lekunutu