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Sehlahisoa se secha sa ho lemoha lefu la likolobe ho tsoa ho Big Fish se se se thakhotsoe. Ho fapana le li-reagents tsa setso tsa ho lemoha mokelikeli tse hlokang ho lokisoa ka letsoho ha sistimi ea karabelo, reagent ena e nka foromo ea microsphere e omisitsoeng ka botlalo, e ka bolokoang mochesong oa kamore ntle le ho ama ts'ebetso ea reagent. Nakong ea ho fumanoa, ke feela nucleic acid e nkiloeng e lokelang ho kenngoa ka ho bula sekoahelo. Ka mor'a hore reagent e qhibilihe ka ho feletseng, e ka lekoa mochine. E kopantsoe le li-reagents tsa ho ntša nucleic acid ka botlalo le lihlahisoa tsa lisebelisoa tse tsoang ho Big Fish, Big Fish e phatlalalitse ka molao tharollo ea metsotso e 40 ea ho lemoha ka potlako bakeng sa mafu a likolobe. Ka merero e meholo e tšeletseng ea ho lemoha ho kenyelletsa Blue Ear, Pseudorabies, Swine Fever, Circovirus, Non Circovirus, le Porcine Influenza, ho nka metsotso e ka bang 40 feela ho phethela ts'ebetso eohle ea PCR fluorescence quantitative discovery ho tloha ho sampuli ho sebetsa ho fihlela liphetho tsa ho lemoha.
Mokhoa oa Tharollo
1. Ho ntšoa ka katleho - lisampole tse ngata li ka sebetsoa ka metsotso e 10
Ka ho sebelisa Big Fish universal nucleic acid extraction le purification reagent e kopantsoeng le sesebelisoa sa ho ntša nucleic acid ka ho feletseng le sesebelisoa sa tlhoekiso, ho tlosoa ha nucleic acid ho ka etsoa ka lisampole tse sa tšoaneng (ho akarelletsa le mali a feletseng, serum, plasma, li-swabs tsa tikoloho, li-swabs tsa molomo, li-fecal swabs, joalo-joalo) nakong ea metsotso e ka bang 10 ntle le tlhokahalo ea sampuli e rarahaneng ea pretreat. Ka mor'a ho kenya mohlala, e ka ntšoa ka mochine.
2. Ho phahamisa ka potlako -30 min e potlakileng ea fluorescence quantification
Ka ho sebelisa Big Fish BFOP-1650 Fluorescence Quantitative PCR Analyzer, mokhoa o hlaha oa mochini oa ho lemoha o omisitsoeng oa Fish Fish o ka kengoa tšebetsong. Motsoako oa li-reagents tse omisang serame le lenaneo la ho lemoha ka potlako la metsotso e 30 ka 'nete li ka fihlela sekoaelo se bulehileng le tlhahlobo ea setšeng.
3. Tlhahlobo e bohlale - ts'ebetso ea bohlokoa, tlhahlobo e ikemetseng
The Big Fish BFOP-1650 fluorescence quantitative PCR analyzer ha e hloke litlhophiso tse rarahaneng tsa lenaneo. Tobetsa "Start" hore lintho tsohle tse sibolloang li qale ho fumanoa. Ka mor'a hore amplification e phethoe, ka boeona e etsa kahlolo e ntle le e mpe ntle le tlhahlobo ea boitsebiso ba letsoho.
Nako ea poso: Jun-21-2025
Litlhophiso tsa lekunutu