PCR Kits: ho fetola tlhahlobo ea lefutso le tlhahlobo ea mafu

Li-PCR (polymerase chain reaction) li fetotse tlhahlobo ea liphatsa tsa lefutso le tlhahlobo ea mafu, li fana ka lisebelisoa tse matla tsa ho holisa le ho sekaseka lisampole tsa DNA le RNA. Lisebelisoa tsena li fetohile karolo ea bohlokoa ea baeloji ea morao-rao ea limolek'hule 'me li ntlafalitse haholo bokhoni ba rona ba ho lemoha le ho ithuta mafu a lefutso, likokoana-hloko tse tšoaetsanoang le mefuta e meng ea lefutso.

Lisebelisoa tsa PCRli etselitsoe ho nolofatsa ts'ebetso ea ho holisa DNA le ho etsa hore e fihlellehe ho bafuputsi ba bangata le litsebi tsa tlhokomelo ea bophelo bo botle. Bokhoni ba PCR ba ho kopitsa tatellano e itseng ea DNA kapele le ka nepo e se e le theknoloji ea bohlokoa mafapheng a fapaneng ho kenyelletsa tlhahlobo ea bongaka, forensics le lipatlisiso.

O mong oa melemo ea mantlha ea lisebelisoa tsa PCR ke ho feto-fetoha ha tsona le ho ikamahanya le maemo lits'ebetsong tse fapaneng. Hore na ho tsebahatsa liphetoho tsa lefutso tse amanang le mafu a futsitsoeng, ho lemoha likokoana-hloko lisampong tsa bongaka, kapa ho sekaseka bopaki ba DNA lipatlisisong tsa botlokotsebe, PCR kits e fana ka mekhoa e tšepahalang le e sebetsang ea ho holisa le ho sekaseka liphatsa tsa lefutso.

Lefapheng la tlhahlobo ea bongaka, lisebelisoa tsa PCR li bapala karolo ea bohlokoa ho lemoheng le ho beha leihlo mafu a tšoaetsanoang. Bokhoni ba ho holisa ka potlako le ho lemoha liphatsa tsa lefutso tsa likokoana-hloko tse kang livaerase le libaktheria li phetha karolo ea bohlokoa tlhahlobong le taolong ea mafu a tšoaetsanoang, ho kenyeletsoa le seoa se ntseng se tsoela pele sa COVID-19. Liteko tse thehiloeng ho PCR li fetohile tekanyetso ea khauta ea ho fumana tšoaetso ea kokoana-hloko ka lebaka la kutloisiso ea bona e phahameng le ho hlaka.

Ntle le moo, lisebelisoa tsa PCR li thusa nts'etsopele ea meriana e etselitsoeng motho ka ho tsebahatsa matšoao a lefutso a amanang le karabelo ea lithethefatsi le ho ba le ts'oaetso ea mafu. Sena se lebisa mekhoeng e mengata ea phekolo e lebisitsoeng le e sebetsang, kaha bafani ba tlhokomelo ea bophelo ba ka fetola mekhoa ea bongaka ho latela boemo ba lefutso la motho.

Tšusumetso ea lisebelisoa tsa PCR e fetela ka nģ'ane ho bophelo ba batho, ka ts'ebeliso ea temo, tlhokomelo ea tikoloho le paballo ea mefuta-futa ea lihloliloeng. Lisebelisoa tsena li thusa ho ithuta ka mefuta-futa ea liphatsa tsa lefutso tsa limela le liphoofolo, ho khetholla likokoana-hloko tse fetotsoeng ka liphatsa tsa lefutso, le ho hlokomela litšila tsa tikoloho.

Ha theknoloji e ntse e tsoela pele, lisebelisoa tsa PCR li ntse li tsoela pele ho fetoha ho fihlela tlhoko e ntseng e hola ea tlhahlobo ea liphatsa tsa lefutso le tlhahlobo ea lefutso. Tsoelo-pele ea nako ea sebele ea PCR (qPCR) e ntlafalitse haholoanyane kutloisiso le lebelo la tlhahlobo ea liphatsa tsa lefutso, e leng ho lumellang palo ea nako ea sebele ea DNA le RNA. Sena se bula menyetla e mecha ea tlhahlobo ea boemo bo holimo le ho lekola sepheo sa liphatsa tsa lefutso ka lisampole tse fapaneng.

Ho feta moo, ho hlaha ha lisebelisoa tsa PCR tse nkehang habobebe le ntlha ea tlhokomelo e atolositse phihlello ea tlhahlobo ea liphatsa tsa lefutso, haholo-holo libakeng tse lekanyelitsoeng tsa lisebelisoa le libakeng tse hole. Lisebelisoa tsena tsa PCR tse nkehang li na le monyetla oa ho tlisa tlhahlobo e tsoetseng pele ea liphatsa tsa lefutso ho batho ba sa bolokehang, e leng ho nolofalletsang ho lemoha kapele le ho kenella ha mafu a lefutso le a tšoaetsanoang.

Ho ea pele, ntlafatso e tsoelang pele le ntlafatso ea lisebelisoa tsa PCR li lebelletsoe ho tsamaisa tsoelopele e eketsehileng litekong tsa liphatsa tsa lefutso le tlhahlobo ea mafu. Ho tloha ho ho ntlafatsa lebelo le ho nepahala ha tlhahlobo ea liphatsa tsa lefutso ho isa ho ho holisa boholo ba lits'ebetso, lisebelisoa tsa PCR li tla tsoela pele ho theha sebopeho sa baeloji ea limolek'hule le meriana e ikhethileng.

Ka kakaretso,Lisebelisoa tsa PCRntle le pelaelo ba fetotse tlhahlobo ea liphatsa tsa lefutso le tlhahlobo ea mafu, ho fa bafuputsi le litsebi tsa tlhokomelo ea bophelo lisebelisoa tse ngata tse matla bakeng sa ho holisa le ho sekaseka liphatsa tsa lefutso. Ha kutloisiso ea rona ea liphatsa tsa lefutso le phello ea eona bophelong ba batho le ho feta e ntse e tsoela pele, lisebelisoa tsa PCR li tla tsoela pele ho ba ka pele ho tlhahlobo ea liphatsa tsa lefutso, ho khanna boqapi le tsoelo-pele lefapheng la baeloji ea limolek'hule.