Protocol bakeng sa ho lemoha tšimoloho ea liphoofolo tsa Bigfish

Bothata ba polokeho ea lijo bo ntse bo mpefala le ho feta. Ha phapang ea theko ea nama e ntse e eketseha butle-butle, ketsahalo ea "ho fanyeha hlooho ea nku le ho rekisa nama ea ntja" e etsahala hangata. Ho belaelloa ka bomenemene ka liphatlalatso tsa bohata le ho hatakela litokelo le lithahasello tse molaong tsa bareki, ho fokotsa setumo sa sechaba sa polokeho ea lijo, se bakang tšusumetso e mpe sechabeng. E le ho netefatsa polokeho ea lijo hantle le tlhahiso ea polokeho ea leruo la mehlape naheng ea rona, litekanyetso le mekhoa e tšepahalang ea tlhahlobo e hlokahala ka potlako.

Ka boqapi bo tsoelang pele le ho phehella ha bafuputsi, Bigfish e ikemetse e ntlafalitse lisebelisoa tsa ho lemoha tse nkiloeng ke liphoofolo, e fana ka litharollo tse tsoetseng pele le tse potlakileng ho bareki ba rona! Hape re ikutloa re le motlotlo haholo ho khona ho thusa bareki ba rona ho rarolla mathata a bona.
Lebitso la sehlahisoa: Setsi sa ho lemoha semelo sa liphoofolo (kolobe, khoho, pere, khomo, nku)
Boikutlo bo phahameng: moeli oa bonyane oa ho lemoha 0.1%
Lintlha tse phahameng: boitsebiso bo nepahetseng ba mefuta eohle ea "nama ea sebele le ea bohata", ha ho na cross-reactivity
1. Mohlala oa ho sebetsa
Mehlala e ile ea hlatsuoa ka makhetlo a mabeli ho isa ho a mararo ka 70% ea ethanol le metsi a qhibilihisitsoeng habeli, a bokelloa ka har'a li-tubes tse hloekileng tsa 50 ml tsa centrifuge kapa mekotla e tiisitsoeng e hloekileng ebe e bolokoa e le leqhoa ho -20 ° C. Mehlala e arotsoe ka likarolo tse tharo tse lekanang, ho kenyelletsa le sampuli e lokelang ho lekoa, sampuli e tsosolositsoeng le sampuli e bolokiloeng.
2. Ho ntšoa ha asiti ea nucleic
Mehlala ea lithane e omisoa ebe e siloa ka botlalo kapa e eketsoa ho naetrojene e metsi, ebe e etsoa phofo ka seretseng le pestle, ebe DNA ea liphoofolo e ntšoa ka mokhoa o iketsang.sesebelisoa sa nucleic acid + Magpure Animal Tissue Genomic DNA Purification Kit.

(Setsi sa tlhahiso ea laboratori)

3. Teko ea ho hōlisa
Teko ea amplification e etsoa ho sebelisoa Bigfish sequential real-time quantitative fluorescence PCR analyzer + e nkiloeng ho liphoofolo ho fumana ka nepo hore na nama e ferekantsoe ho latela liphetho tse mpe, ho sireletsa hamolemo litokelo tsa bareki le polokeho ea lijo.

Mehlala: Letlapa la Tekolo la Tsoho ea Liphoofolo (Linku)