Phetolelo ea PCR: FastCycler Thermal Cycler

Lefapheng la baeloji ea limolek'hule,libaesekele tse chesangke lisebelisoa tsa bohlokoa bakeng sa bafuputsi le bo-ramahlale. Li bapala karolo ea bohlokoa ts'ebetsong ea polymerase chain reaction (PCR), e leng motheo oa ho holisa DNA, cloning le litlhahlobo tse fapaneng tsa lefutso. Har'a libaesekele tse ngata tse futhumatsang 'marakeng, FastCycler e hlahella ka likarolo tsa eona tse tsoetseng pele le likarolo tsa boleng bo holimo, e fetoha mohlala oa boqapi le bokhoni.

Bohareng ba FastCycler ke boitlamo ba eona ba boleng, ho sebelisa likarolo tsa pele tsa Peltier tse tsoang Marlow, USA. Lintho tsena li tumme ka ho ts'epahala le ts'ebetso ea tsona, e nolofalletsang FastCycler ho fihlela litekanyetso tse makatsang tsa mocheso ho fihla ho 6 ° C/S. Bokhoni bona ba ho matha ka potlako bo bohlokoa ho fokotsa nako eohle e hlokahalang bakeng sa ho palama baesekele ea PCR, ho lumella bafuputsi ho fumana liphetho kapele ntle le ho senya botšepehi ba teko.

E 'ngoe ea likarolo tse hlahelletseng tsa FastCycler ke palo ea eona e khahlang ea potoloho, e fetang lipotoloho tse limilione tse 100. Ho tšoarella hona ho bolela hore bafuputsi ba ka sebelisa FastCycler nako e telele, e leng se etsang hore e be letsete le theko e tlaase bakeng sa lilaboratori tse hlokang ho palama libaesekele tse futhumetseng le tse pheta-phetoang. Ho phela halelele ha FastCycler ke bopaki ba moralo oa eona o thata le boenjiniere, ho netefatsa hore e khona ho mamella mathata a ts'ebeliso ea laboratori ea letsatsi le letsatsi.

Ho nepahala ha mocheso ho bohlokoa lits'ebetsong tsa PCR, mme FastCycler e ea ipabola ntlheng ena. Ka theknoloji e tsoetseng pele ea mocheso oa thermoelectric le pholileng e kopantsoeng le taolo ea mocheso ea PID (proportional-integral-derivative), FastCycler e boloka boemo bo phahameng ba mocheso o nepahetseng nakong eohle ea ho palama baesekele. Ho nepahala hona ho bohlokoa bakeng sa ho fihlela ntlafatso e nepahetseng ea DNA, kaha le ho kheloha hanyenyane mochesong ho ka lebisa liphellong tse mpe kapa ho hlōleha ha liteko.

Ho tšoana ho liliba tsohle ke karolo e 'ngoe ea bohlokoa ea ho palama baesekele e futhumetseng,' me FastCycler ha e soetsehe. Moralo oa eona o tiisa hore lisampole tsohle lia futhumatsoa le ho pholile ka mokhoa o tsitsitseng, e leng ntho ea bohlokoa bakeng sa liteko tse hlokang maemo a tšoanang. Ho tšoana hona ho fokotsa ho fetoha ha liphetho, ho fa bafuputsi kholiseho ea hore data ea bona e ka tšeptjoa ebile e ka hlahisoa hape.

Ntle le moo, FastCycler e sebetsa maemong a lerata a tlase, e etsa hore e be e loketseng bakeng sa libaka tsa lab tse hlokang moea o khutsitseng. Karolo ena ha e ntlafatse maemo a ts'ebetso ea bafuputsi feela, empa e boetse e etsa hore boiphihlelo ba lab bo tsepame le ho sebetsa hantle.

Ka kakaretso, theFastCycler Thermal Cyclere emela tsoelopele e kholo ho theknoloji ea PCR. Ka likarolo tsa eona tsa boleng bo holimo tsa Peltier, litefiso tse potlakileng, index ea libaesekele tse ntle haholo, le mochini o tsoetseng pele oa taolo ea mocheso, e etselitsoe ho fihlela litlhoko tsa lipatlisiso tsa sejoale-joale tsa baeloji ea limolek'hule. Hore na o etsa meralo ea ho holisa DNA kapa o etsa lipatlisiso tse rarahaneng tsa liphatsa tsa lefutso, FastCycler e fana ka ts'ebetso e phahameng, e ts'epahalang le e sebetsang hantle. Ho tsetela ho FastCycler ho bolela ho tsetela bokamosong ba lipatlisiso tsa hau, ho netefatsa hore u na le lisebelisoa tseo u li hlokang ho sutumelletsa meeli ea tšibollo ea mahlale.