Ho Fetola PCR: FastCycler Thermal Cycler

Lefapheng la baeloji ea limolek'hule,li-cycli tsa mochesoke lisebelisoa tsa bohlokoa bakeng sa bafuputsi le bo-rasaense. Li bapala karolo ea bohlokoa ts'ebetsong ea karabelo ea ketane ea polymerase (PCR), e leng motheo oa keketseho ea DNA, ho cloning le litlhahlobo tse fapaneng tsa liphatsa tsa lefutso. Har'a li-cyclers tse ngata tsa mocheso tse 'marakeng, FastCycler e ikhetha ka likarolo tsa eona tse tsoetseng pele le likarolo tsa boleng bo holimo, e leng mohlala oa boqapi le bokhoni.

Bohlokwa ba FastCycler ke boitlamo ba yona ba boleng, e sebedisa dielemente tsa Peltier tsa maemo a hodimo tse tswang Marlow, USA. Dielemente tsena di tumme ka ho tshepahala le tshebetso ya tsona, e leng se nolofalletsang FastCycler ho fihlella ditefiso tse makatsang tsa ho phahama ha mocheso ho fihla ho 6°C/S. Bokgoni bona ba ho phahama ka potlako bo bohlokwa ho fokotsa nako yohle e hlokahalang bakeng sa ho potoloha ha PCR, e leng se dumellang bafuputsi ho fumana diphetho kapele ntle le ho beha botshepehi ba teko kotsing.

E 'ngoe ea litšobotsi tse ikhethang tsa FastCycler ke palo ea eona e khahlang ea lipotoloho, e fetang lipotoloho tse limilione tse 100. Ho tšoarella hona ho bolela hore bafuputsi ba ka sebelisa FastCycler nako e telele, e leng se etsang hore e be letsete le theko e tlaase bakeng sa lilaboratori tse hlokang potoloho ea mocheso e tsoelang pele le e pheta-phetoang. Bophelo ba nako e telele ba FastCycler ke bopaki ba moralo oa eona o thata le boenjiniere, ho netefatsa hore e ka mamella mathata a ts'ebeliso ea laboratori ea letsatsi le letsatsi.

Ho nepahala ha mocheso ho bohlokoa haholo lits'ebetsong tsa PCR, 'me FastCycler e ipabola ntlheng ena. Ka theknoloji e tsoetseng pele ea ho futhumatsa le ho pholisa ea thermoelectric e kopantsoeng le taolo ea mocheso ea PID (proportional-integral-derivative), FastCycler e boloka boemo bo phahameng ba ho nepahala ha mocheso nakong eohle ea ts'ebetso ea ho potoloha. Ho nepahala hona ho bohlokoa ho fihlelleng keketseho e ntle ea DNA, kaha esita le ho kheloha hanyane mochesong ho ka lebisa liphellong tse mpe kapa ho hloleheng ha liteko.

Ho tšoana ho pholletsa le liliba tsohle ke karolo e 'ngoe ea bohlokoa ea potoloho ea mocheso, 'me FastCycler ha e soetse. Moralo oa eona o netefatsa hore lisampole tsohle li futhumatsoa le ho pholisoa ka mokhoa o tsitsitseng, e leng sa bohlokoa bakeng sa liteko tse hlokang maemo a tšoanang. Ho tšoana hona ho fokotsa ho fapana ha liphetho, ho fa bafuputsi kholiseho ea hore data ea bona ea tšepahala ebile e ka ikatisa hape.

Ho phaella moo, FastCycler e sebetsa maemong a lerata le tlase, e leng se etsang hore e be ntle bakeng sa libaka tsa laboratori tse hlokang moea o khutsitseng. Tšobotsi ena ha e ntlafatse maemo a mosebetsi a bafuputsi feela, empa e boetse e etsa hore phihlelo ea laboratori e tsepamise maikutlo le ho sebetsa hantle haholoanyane.

Ka bokhutšoanyane,Mochini oa Thermal oa FastCyclere emela kgatelopele e kgolo theknolojing ya PCR. Ka dielemente tsa yona tsa boleng bo hodimo tsa Peltier, ditekanyo tse potlakileng tsa ho rampa, index e ntle ya ho palama baesekele, le mokgwa o tswetseng pele wa taolo ya mocheso, e etseditswe ho fihlela ditlhoko tsa dipatlisiso tsa baeloji ya sejwalejwale ya dimolek'hule. Hore na o etsa keketseho ya DNA ya kamehla kapa o etsa dipatlisiso tse rarahaneng tsa lefutso, FastCycler e fana ka tshebetso e phahameng, botshepehi le bokgoni. Ho tsetela ho FastCycler ho bolela ho tsetela bokamosong ba dipatlisiso tsa hao, ho netefatsa hore o na le disebediswa tseo o di hlokang ho sutumelletsa meedi ya diphuputso tsa saense.