Tataiso ea Bohlokoa ho Li-Tubes tsa 8-Strip PCR: Ho Fetolela Phallo ea Mosebetsi oa Lab ea Hao

Lefapheng la baeloji ea limolek'hule, ho nepahala le ho sebetsa hantle ke tsa bohlokoa ka ho fetisisa. Sesebelisoa se seng se ntlafatsang ts'ebetso ea laboratori haholo ke 8-plex PCR tube. Li-tubes tsena tse ncha li etselitsoe ho nolofatsa tšebetso ea polymerase chain reaction (PCR), ho lumella bafuputsi ho etsa liteko habonolo le ka nepo. Ho blog ena, re tla hlahloba melemo ea li-tubes tsa 8-plex PCR, maemo a ts'ebeliso, le malebela a ho holisa bokhoni ba tsona laboratoring.

Li-tubes tsa PCR tse 8-strip ke eng?

Li-tubes tse 8 tsa PCRli entsoe ka li-tubes tse robeli tse arohaneng tsa PCR tse hokahantsoeng ka letoto ho etsa tube e hlobolang. Moqapi ona o lumella lisampole tse ngata ho holisoa ka nako e le 'ngoe, ho etsa hore e tšoanelehe ka ho khetheha bakeng sa liteko tse phahameng. Mong le e mong PCR tube ka hlobolisa tube ka tšoara e itseng bophahamo ba modumo ea karabelo motsoako, hangata 0,1 di ml ho 0,2 di ml, e leng ke a loketse bakeng sa mefuta e fapaneng ya dikopo PCR.

Melemo ea ho sebelisa li-tubes tsa PCR tse 8-strip

1. Ntlafatsa tshebetso: O mong oa melemo ea bohlokoa ka ho fetisisa ea ho sebelisa li-tubes tsa PCR tse 8 ke ho boloka nako ea ho itokisa. Ho e-na le ho sebetsana le li-tubes tsa PCR ka bomong, bafuputsi ba ka kenya lisampole tse ngata ka nako e le 'ngoe, ho fokotsa kotsi ea tšoaetso le phoso ea batho.
2. Ea moruo le e sebetsang hantle: Ka ho sebelisa li-strip tsa liteko, lilaboratori li ka fokotsa palo ea lisebelisoa tse hlokahalang bakeng sa liteko. Sena ha se fokotse litšenyehelo feela, empa hape se fokotsa tšusumetso ea li-plastiki tse lahliloeng tikolohong.
3. Mohlala o ntlafalitsoeng oa ho latedisa: Li-tubes tse ngata tsa 8-strip PCR li tla le libaka tse tšoailoeng ka ho hlaka, tse lumellang bafuputsi ho khetholla lisampole habonolo. Ts'ebetso ena e bohlokoa litekong moo ho lekoa ha mohlala o nepahetseng ho leng bohlokoa ho etsa bonnete ba hore liteko li sebetsa hape.
4. Automation e tsamaellana: Ha lilaboratori li ntse li tsoela pele ho amohela theknoloji ea othomathike, moralo oa 8-strip PCR tube e boetse e tsamaellana le litsamaiso tsa boiketsetso. Tšebelisano ena e eketsa ts'ebetso mme e ts'ehetsa meralo e rarahaneng ea liteko.
5. Ho feto-fetoha ha maemo: Li-tubes tsa PCR tsa 8-strip li ka sebelisoa bakeng sa mefuta e fapaneng ea lisebelisoa, ho kenyelletsa le palo ea PCR (qPCR), reverse transcription PCR (RT-PCR), le genotyping. Ho feto-fetoha ha eona ho etsa hore e be sesebelisoa sa bohlokoa bakeng sa lilaboratori tse ngata tsa baeloji ea limolek'hule.

Tšebeliso ea 8-strip PCR tube

Ts'ebeliso ea li-tubes tsa PCR tse 8-strip li pharalletse ebile li fapane. Hangata li sebelisoa ho:

• Litlhahlobo tsa bongaka: Lilaboratoring tsa bongaka, li-tubes tsa PCR tse 8 li ka sebelisoa ho lemoha kapele mafu a tšoaetsanoang, mafu a lefutso le matšoao a mofetše.
• Lipatlisiso le nts'etsopele: Litlhophisong tsa lipatlisiso tsa thuto le indasteri, li-tubes tsena li bohlokoa bakeng sa lipatlisiso tsa lefutso, nts'etsopele ea ente, le lits'ebetso tse ling tsa baeloji ea limolek'hule.
• Mahlale a forensic: Bokhoni ba ho holisa DNA ho tsoa lisampoleng tse nyane bo etsa hore li-tubes tse 8 tsa PCR li be bohlokoa liphuputsong tsa forensic, moo bopaki bo bong le bo bong bo leng bohlokoa.

Malebela a ho ntlafatsa ts'ebeliso ea li-tubes tsa PCR tse 8-strip

1. Ntlafatsa maemo a karabelo: Netefatsa hore maemo a PCR a ntlafalitsoe bakeng sa teko ea hau e khethehileng. Sena se kenyelletsa ho lokisa mocheso oa annealing, nako ea katoloso, le khatello ea enzyme.
2. Sebelisa li-reagents tsa boleng bo holimo: Katleho ea PCR e itšetlehile haholo ka boleng ba li-reagents tse sebelisoang. Ke feela ka ho khetha DNA polymerase ea boleng bo holimo, li-primers le buffers moo ho ka fumanoang liphetho tse tšepahalang.
3. Boloka sterility: Ho thibela tšilafalo, kamehla sebelisa mokhoa oa aseptic ha u tšoara li-tubes tsa PCR tse 8-strip. Sena se kenyelletsa ho roala liatlana, ho sebetsa tikolohong e hloekileng, le ho qoba tšoaetso lipakeng tsa lisampole.
4. Poloko e nepahetseng: Boloka li-tubes tsa PCR tse 8 tse sa sebelisoeng sebakeng se phodileng, se omileng ho boloka botšepehi ba tsona. Latela litataiso tsa polokelo ea moetsi.

ha re phethela

Li-tubes tse 8 tsa PCRke thekenoloji e senyang tšimong ea baeloji ea limolek'hule, e nang le melemo e mengata e ka ntlafatsang katleho ea laboratori le ho nepahala. Ka ho utloisisa melemo le ts'ebeliso ea eona, bafuputsi ba ka sebelisa lisebelisoa tsena ho ntlafatsa phallo ea mosebetsi le ho fumana liphetho tse tšepahalang. Hore na o etsa tlhahlobo ea bongaka, lipatlisiso tsa mahlale kapa tlhahlobo ea forensic, ho kenyelletsa li-tubes tse 8 tsa PCR ts'ebetsong ea laboratori ea hau ho ka ntlafatsa haholo ts'ebetso ea hau ea mosebetsi. Amohela bokamoso ba PCR 'me u shebelle liteko tsa hau li atleha ka tharollo ena e ncha!