The Evolution of the Thermal Cycler: Phetoho ho DNA Amplification

Thermal cyclerse fetohile sesebelisoa sa bohlokoahali ho bafuputsi le bo-rasaense lefapheng la baeloji ea limolek'hule le liphatsa tsa lefutso. Mochine ona o mocha o fetotse mokhoa oa ho hōlisa DNA, oa etsa hore e be kapele, e sebetse hantle le ho nepahala ho feta leha e le neng pele. Ho blog ena, re tla hlahloba nts'etsopele ea libaesekele tsa mocheso le phello ea tsona lefapheng la baeloji ea limolek'hule.

Khopolo ea ho palama baesekele e futhumetseng, e kenyelletsang ho futhumatsa khafetsa le ho pholisa motsoako oa karabelo, ke motheo oa polymerase chain reaction (PCR). PCR ke mokhoa o holisang kopi e le 'ngoe kapa tse seng kae tsa DNA ka tatellano e mengata ea boholo, e hlahisa likopi tse likete ho ea ho limilione tsa tatellano e itseng ea DNA. Nts'etsopele ea libaesekele tse futhumatsang e phethile karolo ea bohlokoa ts'ebelisong e atileng le nts'etsopele ea theknoloji ea PCR.

Libaesekele tsa pele tsa mocheso li ne li le ngata 'me li hloka ho lokisoa ha mocheso ka letsoho le ho hlahlojoa khafetsa. Leha ho le joalo, ha theknoloji e ntse e hatela pele, libaesekele tsa sejoale-joale tse futhumatsang mocheso li fetohile lisebelisoa tse tsoetseng pele tse khonang ho laola mocheso ka nepo le ho etsa hore motho a itšepe. Lintlafatso tsena li ekelitse lebelo le bokhoni ba ho holisa DNA haholo, ho lumella bafuputsi ho etsa PCR habonolo le ka ts'epo.

E 'ngoe ea lintlafatso tsa mantlha tsa theknoloji ea libaesekele tse futhumatsang e bile ho hlahisoa ha gradient PCR, e lumellang lithempereichara tse ngata ho lekoa ka nako e le ngoe tekong e le 'ngoe. Karolo ena e ipakile e thusa haholo ho ntlafatsa maemo a PCR bakeng sa template e itseng ea DNA, ho boloka nako le lisebelisoa tsa bafuputsi.

Ho feta moo, ho kopanngoa ha bokhoni ba PCR ba nako ea sebele ho libaesekele tse futhumatsang ho ekelitse ts'ebeliso ea bona le ho feta. PCR ea nako ea sebele, eo hape e tsejoang e le quantitative PCR, e lekola kholo ea DNA ka nako ea nnete, e fana ka lintlha tsa bohlokoa mabapi le palo ea pele ea tatellano ea DNA. Sena se fetotse libaka tse kang tlhahlobo ea polelo ea liphatsa tsa lefutso, genotyping, le ho lemoha pathogen.

The miniaturization ea libaesekele tse futhumatsang e fetohile mokhoa oa bohlokoa lilemong tsa morao tjena, o susumetsoang ke tlhoko ea ho nkeha le ho sebetsa hantle. Libaesekele tsena tse kenelletseng, tse nkehang habobebe tsa libaesekele li fumane lits'ebetso lipatlisisong tsa masimong, tlhahlobong ea tlhokomelo, le maemong a haelloang ke lisebelisoa moo lisebelisoa tsa khale tsa laboratori li ka bang li haellang.

Re shebile pele, bokamoso balibaesekele tse chesange tla bona lintho tse ncha le ho feta. Litheknoloji tse ntseng li hlaha joalo ka PCR ea dijithale le mekhoa ea ho phahamisa isothermal li tlola meeli ea ho holisa DNA le ho fana ka menyetla e mecha ea ho lemoha le ho potlaka ha nucleic acid.

Ka bokhutšoanyane, nts'etsopele ea libaesekele tse futhumatsang e bile le tšusumetso e kholo lefapheng la baeloji ea limolek'hule, e tsamaisang tsoelo-pele ea lipatlisiso, tlhahlobo ea mali le biotechnology. Ho tloha libolokong tsa khale tsa ho futhumatsa ka letsoho ho ea ho lisebelisoa tsa sejoale-joale tse itirisang tse tsoetseng pele, libaesekele tse futhumatsang li fetotse ntlafatso ea DNA, e etsa hore ho be bonolo ho e sebelisa le ho tšepahala ho feta pele. Ha thekenoloji e ntse e tsoela pele, karolo ea libaesekele tse futhumatsang ho bopa bokamoso ba baeloji ea limolek'hule e tla lula e le ea bohlokoa.