Bokamoso ba li-reagents tsa immunoassay: mekhoa le tsoelo-pele

Li-reagents tsa immunoassayphetha karolo ea bohlokoa litlhahlobong tsa bongaka le liphuputsong. Li-reagents tsena li sebelisetsoa ho bona le ho lekanya limolek'hule tse itseng lisampong tsa baeloji, joalo ka liprotheine, lihormone le lithethefatsi. Ha theknoloji e ntse e tsoela pele, bokamoso ba li-reagents tsa immunoassay bo tla bona tsoelo-pele e thabisang le mekhoa e tla ntlafatsa ts'ebetso le bokhoni ba bona.

E 'ngoe ea mekhoa e meholo ea nakong e tlang ea li-reagents tsa immunoassay ke nts'etsopele ea litlhahlobo tse ngata. Multiplexing ka nako e le 'ngoe e ka lemoha bahlahlobisisi ba bangata sampoleng e le' ngoe, e fana ka tlhahlobo e felletseng le e sebetsang hantle. Mokhoa ona o susumetsoa ke tlhoko e ntseng e hola ea tlhahlobo ea boleng bo holimo le tlhoko ea ho boloka molumo oa sampole oa bohlokoa. Ka ho fumana liphofu tse ngata tekong e le 'ngoe, li-multiplex immunoassays li fana ka nako ea bohlokoa le ho boloka litšenyehelo, ho li etsa tse loketseng lipatlisiso le lits'ebetso tsa bongaka.

Mokhoa o mong oa bohlokoa oa nakong e tlang oa li-reagents tsa immunoassay ke kopanyo ea mahlale a macha a ho lemoha. Mekhoa ea setso ea ho itšireletsa mafung hangata e itšetlehile ka mekhoa ea ho lemoha mebala kapa chemiluminescent, e nang le mefokolo ea kutloisiso le mefuta e mengata. Leha ho le joalo, litheknoloji tse ntseng li hlaha tse kang electrochemiluminescence le surface plasmon resonance li fana ka kutlo e phahameng, mefuta e mengata e matla, le bokhoni bo matlafalitsoeng ba ho lemoha ka makhetlo a mangata. Litheknoloji tsena tse tsoetseng pele tsa ho lemoha li lebelletsoe ho fetola li-reagents tsa immunoassay, ho lumella bafuputsi le lingaka ho fumana liphetho tse nepahetseng le tse tšepahalang haholoanyane.

Ho feta moo, bokamoso ba li-reagents tsa immunoassay bo tla tsoela pele ho tsepamisa maikutlo ho ntlafatsa ts'ebetso ea tlhahlobo le ho tiea. Sena se kenyelletsa ho nts'etsapele li-reagents tse nang le botsitso bo boholo, bo ikhethang, le ho ikatisa. Ntle le moo, re sebeletsa ho ntlafatsa liprothokholo tsa tlhahlobo le ho tiisa liforomo tsa tlhahlobo ho netefatsa liphetho tse tsitsitseng le tse tšepahalang lilaboratoring le sethala. Likhatelo-pele tsena li tla thusa ho ntlafatsa ts'epo le boleng ba li-reagents tsa immunoassay, ho li etsa lisebelisoa tsa bohlokoa mefuteng e mengata ea lits'ebetso.

Ntle le tsoelo-pele ea mahlale a morao-rao, bokamoso ba li-reagents tsa immunoassay le tsona li tla susumetsoa ke tlhokahalo e ntseng e hola ea meriana ea motho ka mong le tlhahlobo ea tlhokomelo. Ha indasteri ea tlhokomelo ea bophelo e ntse e fetohela mokhoeng oa motho ka mong le o tsepameng ho mokuli, ho hlokahala li-immunoassay tse ka fanang ka tlhaiso-leseling e potlakileng, e nepahetseng ea tlhahlobo ho ts'ehetsa liqeto tsa bongaka. Mokhoa ona o tsamaisa nts'etsopele ea liforomo tsa immunoassay tse nkehang habonolo le tse bonolo tse ka fanang ka liphetho tsa nako ea nnete sebakeng sa tlhokomelo, ho nolofalletsa ho kenella ka nako le maano a kalafo a motho ka mong.

Ka kakaretso, bokamoso ba li-reagents tsa immunoassay bo khetholloa ka mekhoa e thabisang le tsoelo-pele e tšepisang ho eketsa ts'ebetso ea bona, ho feto-fetoha ha maemo, le phello ea tlhahlobo ea bongaka le lipatlisiso. Ka ho kopanya li-multiplexing, mahlale a tsoetseng pele a ho lemoha, le ho tsepamisa maikutlo ho ntlafatseng ts'ebetso, li-reagents tsa immunoassay li lebelletsoe ho fihlela litlhoko tse tsoelang pele tsa indasteri ea tlhokomelo ea bophelo le ho kenya letsoho ntlafatsong ea meriana e ikhethileng le tlhahlobo ea tlhokomelo. Ha mekhoa ena e ntse e tsoela pele ho fetoha,li-reagents tsa immunoassayntle le pelaelo e tla lula e le sesebelisoa sa bohlokoa ho bo-ramahlale, lingaka le bafani ba tlhokomelo ea bophelo.