Karolo ea bohlokoa ea li-extractors tsa nucleic acid ho biotechnology ea sejoale-joale

Lefapheng le ntseng le hola ka potlako la biotechnology, ho ntšoa ha li-nucleic acid (DNA le RNA) e se e le ts'ebetso ea mantlha bakeng sa lits'ebetso ho tloha ho lipatlisiso tsa lefutso ho isa tlhahlobong ea bongaka. Bohareng ba ts'ebetso ena ke nucleic acid extractor, sesebelisoa sa bohlokoa se nolofatsang ho itšehla thajana ha li-biomolecule tsena tsa bohlokoa ho tsoa mefuteng e sa tšoaneng ea likokoana-hloko. Ho blog ena, re tla hlahloba bohlokoa ba li-extractors tsa nucleic acid, kamoo li sebetsang kateng, le phello ea tsona lipatlisisong tsa saense le tsoelo-pele ea bongaka.

Ho utloisisa li-nucleic acid

Nucleic acids ke litšiea tsa bophelo, tse jereng leseli la lefutso le hlokahalang bakeng sa kholo, nts'etsopele le ts'ebetso ea lintho tsohle tse phelang. DNA (deoxyribonucleic acid) ke moralo oa liphatsa tsa lefutso, ha RNA (ribonucleic acid) e phetha karolo ea bohlokoa ho fetolela boitsebiso ba lefutso ho ba liprotheine. Bokhoni ba ho ntša le ho sekaseka li-nucleic acid tsena bo bohlokoa bakeng sa lithuto tse ngata tsa mahlale tse kang genomics, transcriptomics le tlhahlobo ea limolek'hule.

Bohlokoa ba ho ntša nucleic acid

Ho ntšoa ha Nucleic acid ke mohato oa bohlokoa lits'ebetsong tse ngata tsa laboratori. Ebang e sebelisetsoa ho etsa cloning, tatellano kapa tlhahlobo ea polelo ea lefutso, boleng le bohloeki ba li-nucleic acid tse nkiloeng li ka ama liphetho tsa liteko haholo. Mekhoa e tloaelehileng ea ho ntša, e kang phenol-chloroform extract kapa alcohol precipitation, e ka ba mosebetsi o boima le o jang nako, 'me hangata e lebisa liphellong tse sa tsitsang. Mona ke moo lisebelisoa tsa ho ntša nucleic acid li kenang teng.

Molao-motheo o sebetsang oa sesebelisoa sa ho ntšoa ha acid ea nucleic

Nucleic acid extractorssebelisa mekhoa e fapaneng ea ho arola DNA le RNA liseleng le liseleng. Li-extractors tse ngata tsa morao-rao li sebelisa mekhoa e ikemetseng e kopanyang mehato e mengata ea ts'ebetso ea ho ntša, ho akarelletsa le cell lysis, purification, le elution. Litsamaiso tsena hangata li sebelisa litšiea tse thehiloeng ho silika kapa lifaha tsa makenete ho khetha ka ho khetha li-nucleic acid, ka hona li tlosa litšila tse kang liprotheine le lipids.

Automation ea nucleic acid extract ha e ntlafatse ts'ebetso feela empa hape e fokotsa kotsi ea phoso ea motho, e leng se hlahisang liphello tse tsitsitseng le tse ka hlahisoang hape. Ho phaella moo, lisebelisoa tse ngata tsa ho ntša li-nucleic acid li etselitsoe ho sebetsana le lisampole tse ngata ka nako e le 'ngoe, ho etsa hore e be tse loketseng bakeng sa likopo tse phahameng tsa lipatlisiso le maemo a kliniki.

Likopo tsa lipatlisiso le tsa bongaka

Lisebelisoa tsa li-extractors tsa nucleic acid li pharalletse ebile li fapane. Lilaboratoring tsa lipatlisiso, li-extractors tsa nucleic acid ke lisebelisoa tsa bohlokoa haholo lipatlisisong tsa genomic, tse nolofalletsang bo-ramahlale ho sekaseka phapang ea liphatsa tsa lefutso, ho ithuta tšebetso ea liphatsa tsa lefutso, le ho hlahloba likamano tsa ho iphetola ha lintho. Litsing tsa bongaka, ho ntšoa ha nucleic acid ho bohlokoa bakeng sa ho hlahloba mafu a tšoaetsanoang, mafu a lefutso le mofetše. Bokhoni ba ho ntša ka potlako le ka nepo li-nucleic acid ho lisampole tsa mokuli li lumella ho etsa liqeto tse nakong le tse sebetsang tsa kalafo.

Ho feta moo, ho phahama ha meriana ea motho ka mong ho boetse ho totobalitse bohlokoa ba li-extractors tsa nucleic acid. Ha litlhare tse lebisitsoeng holima liphatsa tsa lefutso tsa motho li hlaha, tlhoko ea li-extractors tsa boleng bo holimo tsa nucleic acid e tla tsoela pele ho hola.

ha re phethela

Ka kakaretso,li-extractors tsa nucleic acidke lisebelisoa tsa bohlokoa lefapheng la biotechnology, tse thusang ho ntša DNA le RNA ka mokhoa o atlehileng le o tšepahalang mefuteng e fapaneng. Tšusumetso ea bona ho lipatlisiso le tlhahlobo ea kliniki e ke ke ea fetisoa, kaha e nolofalletsa bo-rasaense le litsebi tsa tlhokomelo ea bophelo ho senola liphiri tsa genome le ho ntlafatsa liphello tsa mokuli. Ha thekenoloji e ntse e tsoela pele, re ka lebella hore li-extractors tsa nucleic acid li tsoele pele ho fetoha, ho ntlafatsa bokhoni ba tsona le ts'ebeliso ea tsona ho saense ea bophelo. Ebang u mofuputsi, ngaka, kapa motho ea chesehelang saense, ho utloisisa karolo ea li-extractors tsa nucleic acid ke senotlolo sa ho ananela tsoelo-pele e tsotehang e entsoeng lefapheng la biotechnology.