Karolo ea bohlokoa ea li-extract tsa nucleic acid ho biotechnology ea sejoale-joale

Lefapheng le holang ka potlako la theknoloji ea baeloji, ho ntšoa ha li-nucleic acid (DNA le RNA) e se e le mokhoa oa motheo bakeng sa lits'ebetso ho tloha lipatlisisong tsa lefutso ho isa ho tlhahlobo ea bongaka. Motheong oa ts'ebetso ena ke sesebelisoa sa nucleic acid extractor, sesebelisoa sa bohlokoa se nolofatsang ho aroloa ha li-biomolecule tsena tsa bohlokoa ho tsoa lisampoleng tse fapaneng tsa baeloji. Blogeng ena, re tla hlahloba bohlokoa ba li-nucleic acid extractors, kamoo li sebetsang kateng, le tšusumetso ea tsona lipatlisisong tsa saense le tsoelo-pele ea bongaka.

Ho utloisisa li-nucleic acid

Li-nucleic acid ke motheo oa bophelo, li jere tlhahisoleseling ea liphatsa tsa lefutso e hlokahalang bakeng sa kholo, nts'etsopele le ts'ebetso ea lintho tsohle tse phelang. DNA (deoxyribonucleic acid) ke moralo oa lefa la liphatsa tsa lefutso, ha RNA (ribonucleic acid) e bapala karolo ea bohlokoa ho fetoleleng tlhahisoleseling ea liphatsa tsa lefutso hore e be liprotheine. Bokhoni ba ho ntša le ho sekaseka li-nucleic acid tsena bo bohlokoa bakeng sa lithuto tse ngata tsa mahlale tse kang genomics, transcriptomics le tlhahlobo ea limolek'hule.

Bohlokoa ba ho ntšoa ha nucleic acid

Ho ntšoa ha asiti ea nucleic ke mohato oa bohlokoa lits'ebetsong tse ngata tsa laboratori. Ebang e sebelisetsoa ho etsa cloning, sequencing kapa tlhahlobo ea polelo ea liphatsa tsa lefutso, boleng le bohloeki ba li-nucleic acid tse ntšitsoeng li ka ama liphetho tsa liteko haholo. Mekhoa ea setso ea ho ntšoa ha asiti ea phenol-chloroform kapa ho ntšoa ha joala, e ka ba mosebetsi o boima le o jang nako e ngata, 'me hangata e lebisa liphethong tse sa tsitsang. Mona ke moo lisebelisoa tsa ho ntšoa ha asiti ea nucleic li sebetsang teng.

Molao-motheo oa ho sebetsa oa sesebelisoa sa ho ntša nucleic acid

Li-extract tsa nucleic acidsebelisa mekhoa e fapaneng ho arola DNA le RNA liseleng le liseleng. Bongata ba li-extractors tsa sejoale-joale li sebelisa litsamaiso tse iketsang tse kopanyang mehato e 'maloa ea ts'ebetso ea ho ntša, ho kenyeletsoa le ho hlohlelletsa lisele, ho hloekisa le ho hlakisa. Litsamaiso tsena hangata li sebelisa likholomo tse thehiloeng ho silica kapa li-bead tsa makenete ho tlama li-nucleic acid ka mokhoa o ikhethileng, ka hona li tlosa litšila tse kang liprotheine le lipids.

Ho iketsetsa ha ho ntšoa ha nucleic acid ha ho ntlafatse feela bokgoni empa hape ho fokotsa kotsi ya diphoso tsa batho, e leng se fellang ka diphetho tse tsitsitseng le tse ka ikatisang. Ho phaella moo, disebediswa tse ngata tsa ho ntšoa ha nucleic acid di etseditswe ho sebetsana le disampole tse ngata ka nako e le nngwe, e leng se etsang hore di be ntle bakeng sa ditshebediso tse nang le bokgoni bo phahameng dipatlisisong le dibakeng tsa bongaka.

Lipatlisiso le lits'ebetso tsa bongaka

Tšebeliso ea li-extract tsa nucleic acid e pharaletse ebile e fapane. Lilaboratoring tsa lipatlisiso, li-extract tsa nucleic acid ke lisebelisoa tsa bohlokoa lipatlisisong tsa genomic, tse nolofalletsang bo-rasaense ho sekaseka phapang ea liphatsa tsa lefutso, ho ithuta ts'ebetso ea liphatsa tsa lefutso, le ho hlahloba likamano tsa ho iphetola ha lintho. Libakeng tsa bongaka, ho ntšoa ha nucleic acid ho bohlokoa bakeng sa ho hlahloba mafu a tšoaetsanoang, mafu a liphatsa tsa lefutso le kankere. Bokhoni ba ho ntša li-nucleic acid ka potlako le ka nepo lisampoleng tsa bakuli bo lumella liqeto tsa kalafo tse nakong le tse sebetsang.

Ho phaella moo, keketseho ea meriana e etselitsoeng motho ka mong e totobalitse bohlokoa ba li-extract tsa nucleic acid. Ha mekhoa ea phekolo e reretsoeng ho latela liphatsa tsa lefutso tsa motho e ntse e hlaha, tlhoko ea li-extract tsa nucleic acid tsa boleng bo holimo e tla tsoela pele ho hola.

ha re phethela

Ka kakaretso,li-extract tsa nucleic acidke disebediswa tsa bohlokwa lefapheng la theknoloji ya baeloji, tse thusang ho ntsha DNA le RNA ka bokgabane le ka botshepehi disampoleng tse fapaneng. Tshusumetso ya tsona dipatlisisong le tlhahlobong ya bongaka e ke ke ya feteletswa, kaha di thusa bo-rasaense le ditsebi tsa tlhokomelo ya bophelo ho notlolla diphiri tsa genome le ho ntlafatsa diphetho tsa bakudi. Ha theknoloji e ntse e tswela pele ho hatela pele, re ka lebella hore di-extract tsa nucleic acid di tswele pele ho fetoha, di ntlafatse bokgoni ba tsona le tshebediso ya tsona mahlaleng a bophelo. Hore na o mofuputsi, setsebi sa bongaka, kapa morati wa saense, ho utlwisisa karolo ya di-extract tsa nucleic acid ke senotlolo sa ho ananela kgatelopele e makatsang e entsweng lefapheng la theknoloji ya baeloji.