Phetohelo ea Tlhahlobo ea Limolek'hule: Karolo ea Li-Kits tsa Nucleic Acid Extraction

Bohlokoa ba tlhahlobo e tšepahalang ea limolek'hule lefapheng le ntseng le tsoela pele la mahlale a bophelo le tlhokomelo ea bophelo bo botle bo ke ke ba fetisoa. Bigfish e ka pele-pele phetohelong ena, k'hamphani e ikemiselitseng ho tsepamisa maikutlo ho litheknoloji tsa mantlha le ho aha lebitso la khale lebaleng. Ka sepheo sa ho fa bareki lihlahisoa tse tšepahalang tsa tlhahlobo ea limolek'hule, Bigfish e ikemiselitse ho latela mokhoa o thata le o sebetsang oa ho sebetsa le ho ntlafatsa ka mafolofolo ho fihlela litlhoko tsa indasteri.

E 'ngoe ea likarolo tsa bohlokoa tsa tlhahlobo ea limolek'hule ke ho ntšoa ha asiti ea nucleic, e bohlokoa bakeng sa lits'ebetso tse fapaneng, ho kenyelletsa tlhahlobo ea liphatsa tsa lefutso, tlhahlobo ea mafu le lipatlisiso. Lisebelisoa tsa ho ntšoa ha Nucleic acid li bapala karolo ea bohlokoa ts'ebetsong ena, ho thusa bo-ramahlale le litsebi tsa tlhokomelo ea bophelo bo botle ho arola DNA le RNA ka nepo le ka nepo ho mehlala ea baeloji. Bigfish e lemoha boemo bo mahlonoko ba lisebelisoa tsena 'me e entse hore e be ntho ea bohlokoa ho hlahisa litharollo tsa boleng bo phahameng tsa nucleic acid tse finyellang litlhoko tsa lilaboratori tsa morao-rao.

Bohlokoa ba lisebelisoa tsa ho ntša nucleic acid bo holim'a bokhoni ba bona ba ho fana ka li-nucleic acid tse hloekileng le tse sa fetoheng, tse bohlokoa bakeng sa lits'ebetso tse tlase tse kang PCR (polymerase chain reaction), sequencing le cloning. Boleng ba li-nucleic acid tse nkiloeng bo ama ka ho toba ho nepahala le ho tšepahala ha liphetho tse fumanoang lits'ebetsong tsena. Ka hona, ho tsetela ka har'a lisebelisoa tse tsebahalang tsa nucleic acid ho bohlokoa bakeng sa laboratoring efe kapa efe e ikemiselitseng ho fumana liphetho tse tsitsitseng le tse ka hlahisoang hape.

Litlhapi tsa Bigfishlisebelisoa tsa ho ntša asiti ea nucleicli entsoe ho nahanoa ka mosebelisi, li fana ka ts'ebetso e bonolo le e sebetsang e fokotsang nako ea matsoho ha e ntse e eketsa chai le bohloeki. Li-kits li loketse mefuta e fapaneng ea lisampole, ho kenyeletsoa mali, lisele le setso sa lisele, ho li etsa lisebelisoa tse fapaneng bakeng sa lipatlisiso tse fapaneng le lits'ebetso tsa bongaka. Ka ho shebana le mahlale a mantlha, Bigfish e netefatsa hore lisebelisoa tsa eona tsa ho hula li kenyelletsa tsoelo-pele ea morao-rao ea baeloji ea limolek'hule, ho fa bareki litharollo tsa morao-rao tse ntlafatsang bokhoni ba bona ba ho etsa lipatlisiso.

Ho feta moo, boitlamo ba Bigfish ba boqapi bo fetela ka nģ'ane ho ho hlahisa lisebelisoa tsa ho ntša nucleic acid. Khamphani e sebelisana ka mafolofolo le bafuputsi le litsebi tsa tlhokomelo ea bophelo ho utloisisa litlhoko le liphephetso tsa bona, e ba lumellang ho hlophisa lihlahisoa tsa bona ka nepo. Mokhoa ona o shebaneng le bareki ha o hahe tšepo feela, empa hape o beha Bigfish e le moetapele oa tlhahlobo ea limolek'hule.

Ha tlhoko ea tlhahlobo ea limolek'hule e ntse e hola ka tsoelo-pele ea meriana ea motho ka mong le ho ata ha mafu a liphatsa tsa lefutso, karolo ea lisebelisoa tsa ho ntša nucleic acid e tla ba ea bohlokoa le ho feta. Bigfish e itokiselitse ho fihlela tlhoko ena ka ho fana ka litharollo tse tšepahalang, tse sebetsang hantle tse nolofalletsang bafuputsi le lingaka ho etsa liqeto tse nang le tsebo ho latela lintlha tse nepahetseng.

Ka bokhutšoanyane, lisebelisoa tsa ho ntša nucleic acid ke sesebelisoa sa bohlokoahali lefapheng la tlhahlobo ea limolek'hule, 'me Bigfish e ka pele ho popontšo ena. Ka sepheo sa ho aha lebitso la mofuta oa khale, Bigfish e ikemiselitse ho fa bareki lihlahisoa tsa boleng bo holimo tsa tlhahlobo ea limolek'hule tse nang le moea o matla oa ho sebetsa le boqapi bo mafolofolo. Ha k'hamphani e ntse e tsoela pele ho hola le ho holisa lihlahisoa tsa eona, e ntse e tsitsitse boitlamo ba eona ba ho ba k'hamphani ea maemo a lefats'e mafapheng a mahlale a bophelo le tlhokomelo ea bophelo bo botle. Ka ho khetha lisebelisoa tsa Bigfish's nucleic acid extraction, lilaboratori li ka etsa bonnete ba hore li na le lisebelisoa tse molemohali tsa ho ntšetsa pele lipatlisiso tsa tsona le ho ntlafatsa sephetho sa bakuli.