Ho Lokolla Matla a Libaesekele tsa Thermal: Sesebelisoa sa Bohlokoa sa Biotechnology ea Kajeno

Lefapheng la biology ea limolek'hule le biotechnology, libaesekele tse futhumatsang ke lisebelisoa tsa bohlokoahali. Sesebediswa sena seo hangata se bitsoang mochine oa PCR, se phetha karolo ea bohlokoa ho holiseng DNA, e leng se etsang hore e be motheo oa lipatlisiso tsa liphatsa tsa lefutso, tlhahlobo ea mafu le litšebeliso tse fapaneng tsa bongaka le tsa temo. Ho utloisisa tšebetso le bohlokoa ba libaesekele tse futhumatsang mocheso ho ka khantša phello ea tsona tsoelopeleng ea mahlale.

Thermal cycler ke eng?

A sebaesekele se chesangke sesebelisoa sa laboratori se iketsetsang tšebetso ea polymerase chain reaction (PCR). PCR ke mokhoa o sebelisoang ho holisa likarolo tse itseng tsa DNA, e lumellang bafuputsi ho hlahisa limilione tsa likopi tsa tatellano e itseng. Katoloso ena e bohlokoa bakeng sa lits'ebetso tse fapaneng, ho kenyeletsoa cloning, tlhahlobo ea polelo ea liphatsa tsa lefutso, le khatiso ea menoana ea lefutso.
Libaesekele tsa mocheso li sebetsa ka letoto la liphetoho tsa mocheso, tse bohlokoa bakeng sa mekhahlelo e fapaneng ea PCR. Mekhahlelo ena e kenyelletsa denaturation, annealing, le elongation. Nakong ea denaturation, DNA e nang le likarolo tse peli e futhumatsoa, ​​​​e arola likhoele tse peli tse le 'ngoe. Joale mocheso o theoleloa nakong ea mohato oa annealing ho lumella li-primers ho tlama tatellano ea DNA e lebisitsoeng. Qetellong, mocheso oa phahama hape ho kena karolong ea elongation, moo DNA polymerase e kopanyang likhoele tse ncha tsa DNA.

Likarolo tse ka sehloohong tsa sebaesekele se chesang

Libaesekele tsa sejoale-joale tse futhumatsang li na le likarolo tse fapaneng tse ntlafatsang ts'ebetso le ts'ebeliso ea tsona. E 'ngoe ea likhatelopele tsa bohlokoahali ke bokhoni ba ho hlophisa lipotoloho tse ngata tsa mocheso, ho lumella bafuputsi ho etsa liprothokholo tsa bona tsa PCR. Libaesekele tse ngata tsa mocheso li boetse li kenyelletsa li-lids tse futhumetseng tse thibelang condensation ho theha li-tubes tsa reaction, ho netefatsa maemo a nepahetseng bakeng sa ho holisa.
Ntho e 'ngoe e hlokomelehang ke kopanyo ea ts'ebetso ea PCR ea nako ea nnete. Libaesekele tsa nako ea 'nete tsa mocheso li thusa bafuputsi ho lekola ts'ebetso ea ho holisa ka nako ea nnete, ho fana ka lintlha tse ngata mabapi le palo ea DNA e hlahisoang. Karolo ena e bohlokoa haholo lits'ebetsong tse kang quantitative PCR (qPCR), moo litekanyo tse nepahetseng li leng bohlokoa ho fumana liphetho tse nepahetseng.

Tšebeliso ea Thermal Cycler

Lisebelisoa tsa libaesekele tse futhumatsang li pharalletse ebile li fapane. Litlhahlobong tsa bongaka, li sebelisoa ho bona likokoana-hloko, liphetoho tsa lefutso le mafu a futsitsoeng. Mohlala, nakong ea seoa sa COVID-19, libaesekele tse futhumetseng li phethile karolo ea bohlokoa ho lisampole ka potlako, ho thusa ho tseba batho ba tšoaelitsoeng le ho laola ho ata ha vaerase.
Lilaboratoring tsa lipatlisiso, libaesekele tse futhumatsang li bohlokoa bakeng sa ho etsa liphatsa tsa lefutso cloning, tatellano, le lithuto tsa liphatsa tsa lefutso. Li lumella bo-rasaense ho hlahloba phapang ea liphatsa tsa lefutso le ho utloisisa mekhoa ea motheo ea mafu. Ho feta moo, ho tsa temo ea biotechnology, libaesekele tse futhumatsang li sebelisoa ho hlahisa liphatsa tsa lefutso (GMOs) tse ka mamellang khatello ea tikoloho kapa tse nang le phepo e nepahetseng.

Bokamoso ba libaesekele tse futhumatsang

Ha theknoloji e ntse e tsoela pele ho fetoha, ho joalo le ka libaesekele tse futhumatsang. Litlhahiso tse kang miniaturization le kopanyo le sethala sa dijithale li haufi. Likhatelo-pele tsena li lebelletsoe ho etsa hore libaesekele tse futhumatsang li fumanehe habonolo le ho sebelisoa habonolo, ho lumella bafuputsi ho etsa liteko ka katleho e kholo le ho nepahala.
Ho feta moo, ho phahama ha baeloji ea maiketsetso le meriana e etselitsoeng motho ka mong ho ka tsamaisa nts'etsopele e eketsehileng ea theknoloji ea libaesekele tsa mocheso. Ha bafuputsi ba ntse ba batla ho sebelisa liphatsa tsa lefutso ka nepo, tlhoko ea libaesekele tse tsoetseng pele tsa mocheso tse khonang ho ikamahanya le liprothokholo tse rarahaneng e tla eketseha.

ha re phethela

Thesebaesekele se chesang e feta sesebelisoa sa laboratori feela; ke tsela ea ho utloisisa ho rarahana ha bophelo boemong ba limolek’hule. Bokhoni ba eona ba ho holisa DNA bo fetotse maemo ho tloha ho tsa bongaka ho ea ho tsa temo, ho etsa hore e be sesebelisoa sa bohlokoa ho phehelleng tsebo le boqapi bo tsoelang pele. Ha re sheba bokamoso, libaesekele tse futhumatsang ntle le pelaelo li tla tsoela pele ho bapala karolo ea bohlokoa ho bopeng lefapha la biotechnology le lipatlisiso tsa limolek'hule.