Ho Lahla Liphiri tsa Bophelo: Bohlokoa ba Bohlokoa ba Nucleic Acid

Tšimong ea biology ea molek'hule, ntoeng ea li-acid tsa nyutlelie (DNA le Rna) ke mohato oa mantlha, o beha tsela ea tlhahlobo ea liphallelo ho ea tlhahlobo ea liphallelo ho ea tlhahlobo ea liphallelo. Likarolo tsa Nucleic Acid li Fetohile, li etsa hore e sebetse hantle, e tšepahalang ebile e fumaneha ho bafuputsi le laboratori lefatšeng. Blog ena, re tla hlahloba likarolo tsena, bohlokoa ba likarolo tsa bona, le phello ea bona ho ntšetsoa pele tsa mahlale.

Kit

Lisebelisoa tsa nucleic acid kitsMetsoako e etselitsoeng ho arola DNA kapa Rna e tsoang ho mefuta-holo ea bioloji, e kang mali, lisele esita le lisele le tikoloho le tikoloho le tikoloho le eona ea tikoloho. Li-kits tsena hangata li na le bokhopo le li-protocol tsohle tse hlokahalang ho tsamaisa ts'ebetso ea ho tlosa, ho netefatsa bafuputsi ho ka fumana li-acigh tsa boleng bo holimo ka tšilafalo e nyane.

Ts'ebetso ea ho ntšoa

Ts'ebetso e 'ngoe hangata e kenyelletsa mehato ea bohlokoa e mengata ea bohlokoa ka ho itlhoekisa, le ho itlhoekisa le ho itlhoekisa.

Cell LYSIS: Mohato oa pele ke ho bula lisele ho lokolla acid acid. Hangata sena se phethoa ka ho sebelisa sebui sa li-lysis le li-enzyme tse sitisang membrane ea sele le liprotheine tse tlase.

Ho hloekisoa: Kamora hore acid acid acid e lokolloe, mohato o latelang ke ho tlosa li-sinbants tse kang liprotheine tse kang liprotheine, mepress tse ling tsa cellular. Li-kits tse ngata li sebelisa likholomo tsa silita kapa lieta tsa make sa masene ho tlama maqheka a nyutlelie, ka tsela eo a hlatsoang litšila.

TLHOKOMELISO: Qetellong, li-acid tsa Nucleic li hloekisitsoeng li boreleli buffer e loketseng, e se e loketse lits'ebetso tse kang PCR, kapa li koalehile.

Hobaneng o sebelisa Kitcon acid e ntšitsoeng?

Ts'ebetso: Mekhoa ea ho tlosa ha nyele ea nyuc e 'ngoe ea nyutle ea nyutsi e ts'oareha nako le ea sebetsa. Nyeoe ea Nucleic Acid Kits e nolofatsa ts'ebetso mme ka tloaelo e ka qeta nako e fetileng hora.

HO BONAHALA: Lipotoone tse tloaelehileng tse fanoeng ke li-kits tsena li tiisa ho hlahisa tlhahiso le ho ts'epahala ha litholoana. Sena se bohlokoa ho isa litekong moo ho nepahala ho bohlokoa, joalo ka likokoana-hloko tsa bongaka kapa lipatlisiso.

Ho sitisoa ke kite tse ngata ho sebetsana le mefuta e fapaneng ea mehlala, e etsang hore li loketse likopo tse fapaneng. Hore na o sebetsa le disampole tsa motho, lisele tsa bomela, kapa litso tse ka etsahalang ho lumellana le litlhoko tsa hau.

Mesebetsi e nang le botsoalle: Boholo ba li-Nucleic acid bo tla ka litaelo tse qaqileng, le ba ka bang ba ka se be le boiphihlelo bo bongata ba laboratori. Sena se hlahisitse phihlello ea limolecratice ea limoolocuar tsa biology, tse lumellang bafuputsi tse eketsehileng ho nka karolo lipatlisisong tsa lefutso.

Sebetsa sa Nucleic Acid Extraction

Li-acid tsa nyutleric tse fumanoeng li-kits tsena li ka sebetsa e le motheo oa likopo tse ngata:

Patlisiso ea Gene: U utloisisa ts'ebetso ea mofuta, ho fana ka polelo le tsamaiso.
Lintho tsa bongaka tsa bongaka: Ho fumanoa mafu a liphatsa tsa lefutso, mafu a tšoaetsanoang a lefutso, mafu a tšoaetsanoang le mofetše.
Saense ea Forapele: Ho sekaseka sa sampole ea DNA bakeng sa lipatlisiso tsa botlokotsebe.
Biotechnolorature Biotechnogy: Nts'etsopele ea lintho tse phelang tsa lefutso (gmos) ho eketsa chai ea lijalo.
ha re phethela
Lisebelisoa tsa nucleic acid kitske lisebelisoa tsa bohlokoa tsa thuto ea limolek'hule tsa semolek'hule, tse lumella bafuputsi hore ba senole liphiri tsa bophelo maemong a lisele. Ts'ebetso ea bona ea ho fetoha ha tsona, ho feto-fetoha ha bona ho fetotse sebaka sa lipatlisiso tsa liphatsa tsa lefutso le lingaka, ho etsa hore ho be bonolo ho feta neng kapa neng ho etsa hore ho hlahlobe mathata a DNA le Rna. Haholo-holo theknoloji e ntse e tsoela pele, re ka lebella hore li-kit tsena tsa ho fetola lintho tse ling, re bula mamati a macha ka lebaka la ho sibolla saense le ntlafatso ea saense. Hore na u mofuputsi oa boiphihlelo kapa u mocha oa tšimo, tse ntseng li tsetela ka lebaka la tsebo ea nyutlelie ea boleng bo ka ntlafatsang boleng ba mosebetsi oa hau le ho kenya letsoho tsebong e ntseng e eketseha ka linako tse ling tsa lefutso.