Ho notlolla Liphiri Tsa Bophelo: Bohlokoa ba Nucleic Acid Extraction Kits

Lefapheng la baeloji ea limolek'hule, ho ntšoa ha li-nucleic acids (DNA le RNA) ke mohato oa bohlokoa, o bulang tsela bakeng sa likopo tse ngata ho tloha lipatlisisong tsa liphatsa tsa lefutso ho ea ho tlhahlobo ea bongaka. Lisebelisoa tsa ho ntša asiti ea nyutlelie li fetotse tšebetso ena, ea etsa hore e sebetse hantle, e tšepahale, 'me e fumanehe ho bafuputsi le lilaboratori lefatšeng ka bophara. Ka blog ena, re tla hlahloba lisebelisoa tsena, bohlokoa ba likarolo tsa tsona, le phello ea tsona tsoelo-peleng ea mahlale.

Setsi sa ho ntša nucleic acid ke eng?

Lisebelisoa tsa ho ntša asiti ea nucleicke lisebelisoa tse etselitsoeng ho arola DNA kapa RNA ho tsoa mefuteng e fapaneng ea lisampole tsa baeloji, joalo ka mali, lisele, lisele, esita le disampole tsa tikoloho. Li-kits tsena hangata li na le li-reagents le li-protocol tse hlokahalang ho tsamaisa ts'ebetso ea ho ntšoa, ho netefatsa hore bafuputsi ba ka fumana li-nucleic acid tsa boleng bo holimo tse nang le tšilafalo e nyane.

Mokhoa oa ho ntša

Ts'ebetso ea ho ntša hangata e kenyelletsa mehato e 'maloa ea bohlokoa: cell lysis, tlhoekiso, le elution.

Cell Lysis: Mohato oa pele ke ho bula lisele ho lokolla nucleic acid. Hangata sena se finyelloa ho sebelisoa lysis buffer e nang le li-detergents le li-enzyme tse senyang lisele tsa lisele le liprotheine tsa denature.

Tlhoekiso: Ka mor'a hore li-nucleic acid li lokolloe, mohato o latelang ke ho tlosa litšila tse kang liprotheine, lipids le litšila tse ling tsa cellular. Li-kits tse ngata li sebelisa litšiea tsa silika kapa lifaha tsa makenete ho khetha li-nucleic acid, ka hona li hlatsoa litšila.

Elution: Qetellong, li-nucleic acid tse hloekisitsoeng li hlakoloa ka har'a buffer e loketseng, e loketseng lits'ebetso tse tlase tse kang PCR, sequencing, kapa cloning.

Ke hobane'ng ha u lokela ho sebelisa nucleic acid extract kit?

Katleho: Mekhoa e tloaelehileng ea ho ntša li-nucleic acid e nka nako ebile e hloka mosebetsi o boima. Nucleic acid extraction kits e nolofatsa ts'ebetso mme hangata e ka qeta ho ntšoa ka nako ea hora.

Consistency: Litsamaiso tse emeng tse fanoeng ke lisebelisoa tsena li netefatsa hore sephetho sea sebetsa hape le ho tšepahala. Sena se bohlokoa bakeng sa liteko moo ho nepahala ho leng bohlokoa, joalo ka tlhahlobo ea bongaka kapa lipatlisiso.

Mefuta-futa: Lisebelisoa tse ngata li etselitsoe ho sebetsana le mefuta e fapaneng ea sampole, e etsa hore e tšoanele lits'ebetso tse fapaneng. Hore na o sebetsa ka lisampole tsa batho, lisele tsa semela, kapa litloaelo tsa likokoana-hloko, ho ka etsahala hore ho na le lisebelisoa tse lumellanang le litlhoko tsa hau.

Motsoalle oa Mosebelisi: Lisebelisoa tse ngata tsa ho ntša nucleic acid li tla le litaelo tse qaqileng 'me li etselitsoe ho ba bonolo ho li sebelisa, esita le bakeng sa bao e ka 'nang eaba ha ba na phihlelo e pharaletseng ea laboratori. Sena se na le phihlello ea demokrasi ho mekhoa ea baeloji ea limolek'hule, ho lumella bafuputsi ba bangata ho nka karolo lipatlisisong tsa lefutso.

Tšebeliso ea tlhahiso ea nucleic acid

Li-nucleic acid tse fumanoang ho li-kits tsena li ka sebetsa e le motheo oa lits'ebetso tse ngata:

Patlisiso ea Gene: Utloisisa tšebetso ea liphatsa tsa lefutso, polelo le taolo.
Clinical Diagnostics: Ho lemoha mafu a lefutso, mafu a tšoaetsanoang le mofetše.
Saense ea Forensic: Tlhahlobo ea lisampole tsa DNA bakeng sa lipatlisiso tsa botlokotsebe.
Agricultural Biotechnology: Ntlafatso ea liphatsa tsa lefutso (GMOs) ho eketsa chai ea lijalo.
ha re phethela
Lisebelisoa tsa ho ntša asiti ea nucleicke lithulusi tsa bohlokoahali ho baeloji ea sejoale-joale ea limolek'hule, tse lumellang bafuputsi ho senola makunutu a bophelo boemong ba limolek'hule. Bokhoni ba tsona, ho tsitsana le ho feto-fetoha ha tsona ho fetotse boemo ba lipatlisiso tsa liphatsa tsa lefutso le tlhahlobo ea mafu, ho nolofaletsa ho feta leha e le neng pele ho hlahloba ho rarahana ha DNA le RNA. Ha thekenoloji e ntse e tsoela pele, re ka lebella hore lisebelisoa tsena li tsoele pele, li bula mamati a macha a ho sibolloa ha mahlale le boqapi. Ho sa tsotellehe hore na u mofuputsi ea nang le phihlelo kapa u le mocha tšimong, ho tsetela ka thepa ea boleng ba nucleic acid extraction e ka ntlafatsa haholo boleng ba mosebetsi oa hau le ho kenya letsoho molemong oa tsebo e ntseng e eketseha ea liphatsa tsa lefutso.