Mathata a Tloaelehileng ka Thermocyclers ke afe?

 

Mathata a ho Tšoana ha Thempereichara

Bothata bo boholo ka ho fetisisa ba thermocycler bo kenyelletsa ho se tsitse ha mocheso ho pholletsa le boloko. Ho futhumatsa ho sa lekanang ho baka liphetho tse feto-fetohang tsa keketseho, ho beha ts'epo ea liteko kotsing.Mochini oa Thermal oa FastCyclermehlala e sebelisa likarolo tse tsoetseng pele tsa Peltier le li-algorithms tse rarahaneng ho boloka ho tšoana ha ±0.2°C lilibeng tsohle. Leha ho le joalo, li-heating blocks tse tsofalang, peista e senyehileng ea mocheso, kapa lithōle tse bokelletsoeng li ka senya ts'ebetso ha nako e ntse e ea.

Matšoao a mathata a mocheso: Likarabelo tse hlolehileng tsa PCR maemong a itseng a seliba, li-curve tse sa tsitsang tsa ho qhibiliha, kapa chai ea sehlahisoa e feto-fetohang holim'a poleiti e le 'ngoe ea sampole li bontša mathata a ka bang teng a ho tšoana a hlokang ho lekanngoa hang-hang.

Litšitiso tsa ho Futhumatsa ka Sekwahelo

Likoahelo tse futhumetseng li thibela ho kolobisoa ha metsi ho fokotsang metsoako ea karabelo le ho fokotsa katleho ea PCR. Ho hloleha ha ho futhumatsa ha sekwahelo ho har'a litletlebo tse atisang ho hlaha tsa thermocycler. Mocheso o sa lekaneng oa sekwahelo o lumella ho thehoa ha ho kolobisoa ha metsi, athe ho futhumatsa ho feteletseng ho ka senya lisampole kapa ha senya lintho tse sebelisoang tsa polasetiki.

Mekhoa ea sejoale-joale ea FastCycler Thermal Cycler e na le ho futhumatsa sekwahelo se fetoloang (hangata 100-110°C) ka taolo e nepahetseng ea mocheso. Tlhokomelo e tloaelehileng e kenyelletsa ho hlahloba mekhoa ea khatello ea sekwahelo le li-sensor tsa mocheso ho netefatsa hore na ho kopana le ho ajoa ha mocheso ho nepahetse.

Ho senyeha ha sekhahla sa ho phahama

Lebelo la ho phahama ka potlako le khetholla li-thermocycler tsa maemo a holimo ho mehlala ea motheo. Ha nako e ntse e ea, sekhahla sa ho futhumatsa le ho pholisa se ka 'na sa fokotseha ka lebaka la ho senyeha ha likarolo tsa Peltier, ho hloleha ha fene, kapa mathata a sehatsetsing litsamaisong tsa ho pholisa. Ho senyeha hona ho atolosa linako tsa potoloho 'me ho ka ama liketso tse amang mocheso.

Lisebelisoa tsa FastCycler Thermal Cycler tsa boemo ba profeshenale li boloka ho potlaka ka potlako (4-5°C/motsotsoana) ka har'a li-array tse peli tsa Peltier le taolo e ntlafalitsoeng ea mocheso. Ha u reka, netefatsa litlhaloso tsa ho futhumatsa le ho pholisa, eseng feela sekhahla se phahameng sa ho potlaka.

Mathata a Software le Kgokelo

Li-thermocycle tsa sejoale-joale li kopanya software e rarahaneng bakeng sa mananeo a protocol, ho boloka data, le khokahano ea marang-rang. Mathata a tloaelehileng a software a kenyelletsa:

Liphoso tsa firmware: E baka ho senyeha ha lenaneo kapa lipalo tse fosahetseng tsa mocheso

Ho hloleha ha USB/Ethernet: Ho thibela phetiso ya data kapa ho beha leihlo hole

Mathata a skrineng sa ho ama: Ho thatafatsa mananeo a protocol

Mathata a ho tsamaellanaKa litsamaiso tsa taolo ea tlhahisoleseling ea laboratori (LIMS)

Bahlahisi ba etellang pele ba fana ka lintlafatso tsa firmware khafetsa le tšehetso ea tekheniki e arabelang ho rarolla mathata ana kapele.

Ho Tloha le ho Hahola ha Mechini

Likarolo tsa 'mele li senyeha butle-butle:

Tšilafalo ea li-block: Disampole tse tsholotsweng di baka kgokagano e sa lekanang ya mocheso e hlokang ho hlwekiswa ka botlalo

Tšenyo ea lehinge la sekoahelo: Ho buloa khafetsa ho fokolisa likarolo tsa mechini

Ho hloleha ha balateli: Ho fokotsa bokgoni ba ho phodisa le ho eketsa dinako tsa potoloho

Sensor e hoholeha: Ho baka lipalo tse fosahetseng tsa mocheso tse hlokang ho hlahlojoa bocha

Ho Tsamaisa Tekanyo

Li-thermocycle tsohle li hloka netefatso ea tlhahlobo nako le nako. Li-sensor tsa mocheso lia tsamaea ka tlhaho ha nako e ntse e ea, e leng se ka bakang liphoso tse kholo tsa liteko. Lilaboratori tsa litsebi li lokela ho etsa liteko tsa tlhahlobo ea tlhahlobo kotara ka ho sebelisa li-thermometer tsa litšupiso tse netefalitsoeng.

BolengMochini oa Thermal oa FastCyclermehlala e kenyelletsa likarolo tsa ho itlhahloba tse lemosang basebelisi ka litlhoko tsa ho lekanya pele mathata a ama liphetho. Litsamaiso tse ling tse tsoetseng pele li fana ka liprothokholi tsa ho lekanya ka boiketsetso tse fokotsang ho kenella ka letsoho.

Maano a Tlhokomelo ea Thibelo

Fokotsa mathata a thermocycler ka ho e hlokomela ka hloko:

• Hloekisa li-heating blocks khoeli le khoeli u sebelisa li-solvent tse loketseng
• Netefatsa ho nepahala ha mocheso kotara ka di-probe tse lekantsweng
• Ntlafatsa firmware khafetsa ho fumana lintlafatso le litokiso tsa liphoso
• Nka sebaka sa lintho tse sebelisoang (li-gasket tsa sekoahelo, li-pad tsa thermal) ho latela kemiso ea moetsi
• Boloka moea o kenang hantle haufi le lisebelisoa bakeng sa ho pholisa hantle

Ho Khetha Lisebelisoa tse Tšepahalang

Ha o reka li-thermocycler, ela pele bahlahisi ba fanang ka:

Litiisetso tse felletseng: Ho koahela likarolo le mosebetsi

Tšehetso ea tekheniki e arabelang: Ka ho fumaneha ha likarolo tse ncha ka potlako

Rekoto e pakiloeng: Ho bontšitsoe botšepehi lilaboratoring tsa lithaka

Tlhokomelo e bonolo ho e sebelisaLikarolo tse fumanehang habonolo le litokomane tse hlakileng tsa tšebeletso

Qetello

Leha li-thermocycler li ka hlahisa liphephetso tse fapaneng tsa ts'ebetso, ho utloisisa mathata a tloaelehileng ho matlafatsa khetho ea lisebelisoa tse nang le tsebo le moralo o nepahetseng oa tlhokomelo. Ho tsetela lits'ebetsong tsa boleng bo holimo tsa FastCycler Thermal Cycler tse nang le meralo ea motheo e matla ea tšehetso ho fokotsa nako ea ho se sebetse 'me ho netefatsa liphetho tse tsitsitseng le tse tšepahalang tsa PCR. Lekola litšenyehelo tsohle tsa beng ba thepa - ho kenyeletsoa litlhoko tsa tlhokomelo le boleng ba tšehetso - ho fapana le theko ea theko feela. Thermocycler e nepahetseng e ba molekane ea tšepahalang oa laboratori ea fanang ka lilemo tsa ts'ebetso e se nang mathata le liphetho tsa saense tse ka ikatisang.