Sete ea ho Fumana Asiti ea Nyutlelie ea SARS-CoV-2 (Fluorescence RT-PCR)

Tlhaloso e Khutšoanyane:

Sete ea ho lemoha nucleic acid ea SARS-COV-2 (Fluorescence RT-PCR) e ka sebelisoa bakeng sa ho lemoha nucleic acid ea coronavirus e ncha, e sebelisetsoang tlhahlobo e thusang le lipatlisiso tsa epid-emiological tsa tšoaetso e ncha ea coronavirus, e loketseng CDC, lipetlele, laboratori ea bongaka ea motho oa boraro, setsi sa tlhahlobo ea 'mele le lilaboratori tse ling tsa kliniki.

ITEANYE LE RONA

Qaqiso ea Sehlahisoa

Li-tag tsa Sehlahisoa

Likarolo tsa Sehlahisoa

1, Ho ba le kutlo e phahameng: Moeli oa ho Lemoha (LoD)＜Likopi tse 2 × 102/ml.

2, Jini e nang le sepheo se habeli: Lemoha jini ea ORFlab le jini ea N ka nako e le 'ngoe, latela molao oa WHO.

3, E loketse lisebelisoa tse fapaneng: ABI 7500/7500FAST; Roche LightCycler480; BioRad CFX96; BigFish-BFQP96/48 ea rona.

4, E potlakile ebile e bonolo: Reagent e kopantsoeng esale pele e bonolo ho e sebelisa, bareki ba hloka feela ho eketsa enzyme le template. Sete ea Bigfish ea ho ntša nucleic acid e tsamaellana hantle le teko ena. Ka ho sebelisa mochini o felletseng oa ho ntša o ikemetseng, ho potlakile ho sebetsana le lisampole tse ngata..

5, Polokeho ea Bio: Bigfish e fana ka Mokelikeli o Bolokang Mohlala ho tima vaerase kapele ho netefatsa polokeho ea basebetsi.