Ultramicrospectrophotometer

Kenyelletso ea Sehlahisoa

Ultramicrospectrophotometer ke mofuta oa ho lemoha ka potlako le ho nepahetseng ha nucleic acid, protheine le cell solution concentration ntle le preheating, boholo ba sampole feela 0.5 ho 2ul, 'me mokhoa oa cuvette o ka lemoha mahloriso a libaktheria le litaba tse ling tsa setso. Ts'ebetso ea ho lemoha ha Fluorescence e ka penta ka lisebelisoa tsa tlhahlobo ea fluorescence quantitative, ka motsoako o ikhethileng oa lidae tsa fluorescent le lintho tse shebiloeng li ka lekanya ka nepo likhakanyo tsa DNA, RNA le protheine, 'me bonyane bo ka fihla ho 0.5pg/μl (dsDNA).

Likarolo tsa sehlahisoa

Leseli la flicker flicker frequency e khutšoane, ha e bapisoa le mokhoa oa khale oa ho lemoha ho eketsa bophelo ba ts'ebeletso ea mohloli oa leseli. Khothatso ea matla a bobebe a lihlahisoa tse nyane tsa tlhahlobo e ka bonoa kapele, ha ho bonolo ho e theola;

Mosebetsi oa Fluorescence: Ka fluorescence quantitative reagent e ka bona pg concentration dsDNA;

Theknoloji ea ho lemoha tsela ea 4: theknoloji e ikhethang ea ho laola likoloi, tšebeliso ea "4" mokhoa oa ho lemoha tsela ea "4", botsitso, ho pheta-pheta, ho lekana ho molemo, tekanyo ea tekanyo e kholoanyane.;

Mohatisi o hahiloeng ka hare: Ka likhetho tse bonolo tsa ho sebelisa data-to-printer, o ka hatisa litlaleho ka kotloloho ho tsoa khatisong e hahelletsoeng kahare.r;

Tharollo ea baktheria ea OD600, ho lemoha likokoana-hloko: e nang le OD600 ea mokhoa oa ho lemoha tsela ea mahlo, ho feta mokhoa oa lijana o bonolo bakeng sa ho lemoha libaktheria, likokoana-hloko le mekhoa e meng ea setso.;

Pheta-phetoho e phahameng le linearity;