Ultramicrospectrophotometer

Selelekela sa Sehlahisoa

Ultramicrospectrophotometer ke mofuta oa ho lemoha ka potlako le ka nepo ha nucleic acid, protheine le tharollo ea sele ntle le ho futhumatsa pele, boholo ba sampole ke 0.5 ho isa ho 2ul feela, 'me mokhoa oa cuvette o ka lemoha mahloriso a libaktheria le mecha e meng ea litaba ea setso. Mosebetsi oa ho lemoha khanya o ka kopanngoa le kit ea tlhahlobo ea bongata ba khanya, ka motsoako o itseng oa lidae tsa khanya le lintho tse reriloeng li ka lekanya ka nepo mahloriso a DNA, RNA le protheine, 'me bonyane bo ka fihla ho 0.5pg/μl (dsDNA).

Likarolo tsa sehlahisoa

Maqhubu a ho fifala ha mohlodi wa lesedi a makgutshwane, ha a bapiswa le mokgwa wa setso wa ho lemoha ho eketsa bophelo ba tshebeletso ya mohlodi wa lesedi. Ho kgothatsa matla a lesedi la dihlahiswa tse nyane tsa teko ho ka lemoha kapele, ha ho bonolo ho ho senya.;

Mosebetsi oa khanya: Ka khanya ea khanya, reagent ea bongata e ka lemoha mahloriso a pg dsDNA;

Theknoloji ea ho lemoha litsela tse 4 tsa mahlo: theknoloji e ikhethang ea taolo ea enjene, tšebeliso ea mokhoa oa ho lemoha litsela tse 4 tsa mahlo, botsitso, ho pheta-pheta, ho lekana ho betere, sebaka sa ho lekanya se seholo;

Printa e kentsweng kahare: Ka dikgetho tse bonolo ho sebedisa data-to-printer, o ka printa ditlaleho ka kotloloho ho tswa ho printa e kentsweng kaharer;

Tharollo ea baktheria ea OD600, ho lemoha likokoana-hloko: ka sistimi ea ho lemoha tsela ea optical ea OD600, mokhoa oa sejana o loketse libaktheria, likokoana-hloko le ho lemoha mahloriso a tharollo ea setso se seng.;

Ho pheta-pheta ho hoholo le ho lekana hantle;